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Calcitonin ELISA Kit

  • Created on the 1 February, 2017.

BACKGROUND

Calcitonin (CT) is a 32 amino acid peptide hormone which is produced and secreted from the para-follicular C-cells of the thyroid gland and its function is to oppose the action of parathyroid hormone (PTH), this helps to regulate bloods phosphate and calcium levels. Its function is carried out by inhibiting osteoclasts activity, these cells are responsible for breakdown of bone. This allows calcitonin to lessen that amount of calcium that is present in the blood, the secretion of this CT hormone is directly controlled by the blood’s calcium levels. Various forms of calcitonin can be detected in blood samples, for example monomer, dimer, oxidized monomer, precursor of CT and higher molecular weight forms. The concentrations of these peptides is found to vary depending on the clinical status, renal function and normal or ectopic production of CT.

Medullary carcinoma of the thyroid (MTC) is one of the most prominent clinical syndrome that is linked to a disordered hypersecretion of calcitonin, even though it is rare representing 5-10% of all thyroid cancers, it can be quite fatal in many cases. Immunoassay for calcitonin using serum samples have been helpful in diagnosing the presence of MTC providing exceptional degree of specificity and accuracy. It is recommend that the monomer form of CT is detected in this ELISA assay.

INTENDED USE

Calcitonin ELISA kit is a reliable quantitative procedure for measuring calcitonin (CT) in human serum or plasma. This assay has a sensitivity detection limit of 1.3 pg/ml.

SENSITIVITY

The minimum sensitivity detection limit of human calcitonin (CT) using this calcitonin ELISA kit was approximately 1.3 pg/ml. The dynamic assay range for this kit is 7.5 – 573.0 pg/ml.

REFERENCES

  1. Plasma calcitonin as a marker of disease activity in patients with small cell carcinoma of the lung. J. Clin. Endocrinol. Metab. (1981) 53, 3: 602-606. Wallach S.R., Royston I, Taetle R., Wohl H. and Deftos L.
  2. Abnormal calcitonin basal levels and pentagastrin response in patients with chronic renal failure on maintenance hemodialysis. Eur. J. Endocrinol. (1995) 132, 1, 75-81. Nicoli P., et al.
  3. Calcitriol corrects deficient calcitonin secretion in the Vit. D deficient elderly. J. Bone Miner Res. (1994) 9, 1, 53-57. Quesada J.M., et al.
  4. Influence of age and sex on plasma calcitonin in human beings. N. Engl. J. Med. (1980) 302: 1351-1353. Deftos, L.J., et al.
  5. Estimates of circulating monomeric calcitonin: physiological studies in normal and thyroidectomized man. J. Clin. Endocrinol. Metab. (1983) 57: 897. Body J.J. and Heath III, H.

ADDITIONAL INFORMATION

  • Full Name: Calcitonin ELISA Kit
  • Reactivity: Human
  • Sample Type: Serum, Plasma
  • Sensitivity: 1.3 pg/ml

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