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C1q Antibody ELISA Kit

  • Created on the 26 May, 2017.

BACKGROUND

Component C1q is a vital first step of the complement activation pathway, in fact it is one of the central molecules involved. This process involves the globular heads of C1q binding to the Fc regions of immunoglobulins IgG or IgM which can lead the subsequent induced activation of other sub-components of C1, C1r or C1s. C1q is multivalent for attaching to the complement fixation sites of immunoglobulin and these sites are present on the CH4 domain of IgM or on the CH2 domain of IgG. It contains 18 peptide chains in total (3 subunits of 6 peptides) forming a 400 kDa protein. Each subunits is made up of a Y-shaped pair of triple peptide helices that are joined at the stem and end with a globular non-helical head. Anti-C1q autoantibodies are found to be linked to a number of different autoimmune, renal illnesses, systemic lupus erythematosus, lupus nephritis and rheumatic diseases.

INTENDED USE

Human C1q ELISA kit is a method for determining in vitro quantitative amounts of IgG class autoantibodies against component C1q antibody in human serum and plasma samples. This assay has a minimum analytical sensitivity limit of 0.5 U/ml.

SENSITIVITY

The minimum detection sensitivity level of human component C1q antibody (C1q-Ab) using this human C1q ELISA kit was 0.5 U/ml. The dynamic assay range for this kit is 6.3 – 100.0 U/ml.

REFERENCES

  1. C1q, antibodies and anti-C1q autoantibodies. Mol Immunol. (2015) 68 (1): 6-13. Review. Beurskens F.J., et al.
  2. How antibodies use complement to regulate antibody responses. Mol Immunol. (2014) 61 (2): 79-88. Review. Sörman A., et al.
  3. New approaches for detecting complement-fixing antibodies. Curr Opin Organ Transplant. (2012) 17 (4): 409-15. Review. Tyan D.B.
  4. The classical and regulatory functions of C1q in immunity and autoimmunity. Cell Mol Immunol. (2008) 5 (1): 9-21. Review. Lu J.H., et al.

ADDITIONAL INFORMATION

  • Full Name: C1q Antibody ELISA Kit
  • Reactivity: Human
  • Sample Type: Serum, Plasma
  • Sensitivity: 0.5 U/ml

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