- Created on the 9 February, 2017.
Human C-Peptide ELISA kit is a reliable quantitative procedure for measuring connection peptide (C-Peptide) in human serum, plasma and urine. This assay has a minimum sensitivity detection limit of 0.064 ng/ml.
C-Peptide is short for connection peptide because it is responsible for joining the A and B chains of insulin within the proinsulin molecule. The enzymatic cleavage of proinsulin results in the release of insulin into circulation and C-Peptide (a 3000 MW residual fragment) being formed. Human C-Peptide has no metabolic function and it is a 31 amino acid peptide residue that has a half-life in circulation that can be between 2-5 times longer when compared to insulin.
One of the main reasons why there has been great interest from scientist in measuring C-Peptide is because of the fact that both insulin and C-Peptide are secreted in equimolar amounts, therefore by measure C-Peptide levels permits the quantitation of insulin secretion. There are many advantages it offers over measuring insulin such as serum and urinary samples can be measured, displays relative metabolic inertness, between two and five times longer half-life, 5-6 times greater levels in peripheral venous blood and its ability to distinguish endogenous from injected insulin.
Therefore, the measurement of C-Peptide levels is in fact better in many ways to determine the concentration of endogenous insulin secretion than measuring peripheral insulin levels itself. Coupled with the fact that there are highly sensitive assay’s that are available and this will allow the measurement of extremely low concentration possible. The clinical indications for C-Peptide measurement include differentiation from factitious hypoglycaemia, diagnosis of insulinoma, permit evaluation of insulin dependence in maturity onset diabetes mellitus, follow-up of pancreatectomy and evaluation of viability of islet cell transplants.
Reagents which are supplied with the C-Peptide ELISA kit, please following instructions for storage conditions.
1. Microtiterwells: coated with anti-mouse antibody.
2. Standards (0-5): lyophilized, concentrations ranging from 0 – 16 ng/ml.
3. Sample Diluent.
4. Antiserum: monoclonal mouse anti C-Peptide antibody.
5. Enzyme Conjugate.
6. Enzyme Complex.
7. Substrate Solution.
8. Wash Solution.
9. Stop Solution.
The minimum sensitivity detection limit of connection peptide using current human C-Peptide ELISA kit was approximately 0.064 ng/ml. The dynamic range for this assay is 0.2 – 16.0 ng/ml.
- Evidence for inhibitory autocrine effects of proinsulin C-peptide on pancreatic β-cell function and insulin secretion. Diabetes Obes Metab. (2014) 16 (10): 937-46. McKillop A.M., et al.
- C-peptide: new findings and therapeutic possibilities. Diabetes Res Clin Pract. (2015) 107 (3): 309-19. Review. Wahren J. and Larsson C.
- Residual C-peptide in type 1 diabetes: what do we really know? Pediatr Diabetes. (2014) 15 (2): 84-90. Review. VanBuecken D.E. and Greenbaum C.J.
- C-peptide replacement therapy as an emerging strategy for preventing diabetic vasculopathy. Cardiovasc Res. (2014) 104 (2): 234-44. Review. Bhatt M.P., Lim Y.C. and Ha K.S.
- Full Name: C-Peptide ELISA Kit
- Reactivity: Human
- Sample Type: Serum, Plasma, Urine
- Sensitivity: 0.064 ng/ml