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Brucella Abortus IgM ELISA Kit

  • Created on the 12 July, 2017.

BACKGROUND

Brucella are non-motile, rod-shaped, gram-negative bacteria which can infect a number of different livestock such as cattle, elk and bison (B. abortus), dogs (B. canis), sheep and goats (B. melitensis) and pigs and caribou (B. suis). They can cause chronic disease which can in most cases persist for life, by functioning as facultative intracellular parasites. Brucellosis is the disease which is recognised in humans and animals and humans that is caused by this bacterium. B. abortus is the principal cause of brucellosis in cattle and the bacteria can be shed from infected animal during the time of calving or abortion. Some of the most common clinical signs of cattle being infected with B. abortus are retained placenta, high incidences of abortions and arthritic joints.

INTENDED USE

Human Brucella abortus-IgM ELISA kit is designed for analysing in-vitro quantitative levels of human IgM antibodies to Brucella abortus in samples of serum and plasma. This assay has a minimum analytical sensitivity limit of 1.03 U/ml.

SENSITIVITY

The minimum detection sensitivity level of human Brucella abortus IgM using this human Brucella abortus-IgM ELISA kit was 1.03 U/ml. The dynamic assay range for this kit is 1.0 – 200.0 U/ml.

REFERENCES

  1. Further evidence that bovine IgM does not fix guinea pig complement. Vet Immunol Immunopathol. (1988) 19 (3-4): 197-204. Nielsen K. and Duncan J.R.
  2. Detection of IgM and IgG antibodies against Brucella by dot-ELISA in humans. J Commun Dis. (1994) 26 (1): 1-5. Barbuddhe S.B., et al.
  3. Radioimmunoassay of IgM, IgG, and IgA Brucella antibodies. Lancet. (1977) 1 (8021): 1075-8. Parratt D., et al.
  4. Evaluation of ELISA in the diagnosis of acute and chronic brucellosis in human beings. J Hyg (Lond). (1986) 97 (3): 457-69. Araj G.F., et al.
  5. Isolation and purification of bovine IgM by dissociating immunoglobulin-brucella complexes. J Immunol Methods. (1979) 31 (3-4): 379-87. Stone S.S., et al.

ADDITIONAL INFORMATION

  • Full Name: Brucella Abortus IgM ELISA Kit
  • Reactivity: Human
  • Sample Type: Plasma, Serum
  • Sensitivity: 1.03 U/ml

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