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Brucella Abortus IgG ELISA Kit

INTENDED USE

Human Brucella abortus IgG ELISA kit is a procedure intended for detecting in-vitro quantitative concentrations of human IgG antibodies against Brucella abortus (Brucella IgG) in plasma or serum. This assay has a minimum analytical sensitivity limit of 1.11 U/ml.

BACKGROUND

Brucella abortus is a bacterium which is found in cattle and it is gram-negative, a zoonotic bacteria which means that it can easily be transferred from an animal to human and still retain its pathogenic property. B. abortus causes a disease which is called brucellosis, this should always be considered in all abortions, in particular where there are multiple long term abortions in herds. Serology testing can be used for the initial presumptive diagnosis of brucellosis or even to screen herds. Antigen based assay is most likely one of the most common serological methods that tends to be used frequently since the sensitivity of an ELISA test is 100% when compared to blood culture. B. abortus can be eradicated from a herd by a combination of test and removal procedures or in some cases by depopulation.

BRUCELLA IGG ELISA KIT CONTENT

All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.

  • Brucella antigen coated microtiter strips.
  • Enzyme Conjugate.
  • Sample Diluent.
  • TMB Substrate.
  • Calibrator D (Positive Control).
  • Calibrator C (Weak Positive Control).
  • Calibrator A (Negative Control).
  • Calibrator B (Cut-Off Standard).
  • Washing Buffer (10x).
  • Stop Solution.

SENSITIVITY

The minimum detection sensitivity level of IgG antibodies against Brucella abortus (Brucella-IgG) using current human Brucella IgG ELISA kit was 1.11 U/ml. The dynamic range for this assay is 1.0 – 150.0 U/ml.

ASSAY CHARACTERSTICS

– Brucella ELISA: IgG
– Intra-Assay-Precision: 8.7 %
– Inter-Assay-Precision: 7.9 %
– Inter-Lot-Precision: 4.1 – 8.8 %
– Analytical Sensitivity: 1.11 U/mL
– Recovery: 82 – 96 %
– Linearity: 80 – 115 %
– Cross-Reactivity: No cross-reactivity to Bordetella pertussis
– Interferences: No interferences to bilirubin up to 0.3 mg/mL, hemoglobin up to 8.0 mg/mL and triglycerides up to 5.0 mg/mL
– Clinical Specificity: 100 %
– Clinical Sensitivity: 100 %

REFERENCES

  1. Immunoglobulin G binding activity of Brucella abortus. Mol Immunol. (1991) 28 (1-2): 35-9. Bricker B.J., et al.
  2. [Serological diagnosis of brucellosis in cattle. A comparison between ELISA (IgG) and classical serological tests]. Dev Biol Stand. (1984) 56: 451-63. French. Trap D. and Gaumont R.
  3. Evaluation of indirect enzyme-linked immunosorbent assays and IgG avidity assays using a protein A-peroxidase conjugate for serological distinction between Brucella abortus S19-vaccinated and -infected cows. Clin Vaccine Immunol. (2010) 17 (4): 588-95. Pajuaba A.C., et al.
  4. Serological follow-up of human brucellosis by measuring IgG antibodies to lipopolysaccharide and cytoplasmic proteins of Brucella species. Clin Infect Dis. (1996) 22 (3): 446-55. Baldi P.C., et al.
  5. Evaluation of the enzyme-linked immunosorbent assay in the detection of cattle infected with Brucella abortus. Vet Microbiol. (1984) 10 (1): 23-32. Sutherland S.S.

ADDITIONAL INFORMATION

  • Full Name: Brucella Abortus IgG ELISA Kit
  • Reactivity: Human
  • Sample Type: Serum, Plasma
  • Sensitivity: 1.11 U/ml

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