GET A QUOTE

Brucella Abortus IgG ELISA Kit

  • Created on the 12 July, 2017.

BACKGROUND

Brucella abortus is a bacterium which is found in cattle and it is gram-negative, a zoonotic bacteria which means that it can easily be transferred from an animal to human and still retain its pathogenic property. B. abortus causes a disease which is called brucellosis, this should always be considered in all abortions, in particular where there are multiple long term abortions in herds. Serology testing can be used for the initial presumptive diagnosis of brucellosis or even to screen herds. ELISA assay is most likely one of the most common serological methods and the sensitivity of an ELISA test is 100% when compared to blood culture. B. abortus can be eradicated from a herd by a combination of test and removal procedures or in some cases by depopulation.

INTENDED USE

Human Brucella abortus-IgG ELISA kit is a procedure intended for detecting in-vitro quantitative concentrations of human IgG antibodies against Brucella abortus in plasma or serum. This assay has a minimum analytical sensitivity limit of 1.11 U/ml.

SENSITIVITY

The minimum detection sensitivity level of human Brucella abortus IgG using this human Brucella abortus-IgG ELISA kit was 1.11 U/ml. The dynamic assay range for this kit is 1.0 – 150.0 U/ml.

REFERENCES

  1. Immunoglobulin G binding activity of Brucella abortus. Mol Immunol. (1991) 28 (1-2): 35-9. Bricker B.J., et al.
  2. Evaluation of indirect enzyme-linked immunosorbent assays and IgG avidity assays using a protein A-peroxidase conjugate for serological distinction between Brucella abortus S19-vaccinated and -infected cows. Clin Vaccine Immunol. (2010) 17 (4): 588-95. Pajuaba A.C., et al.
  3. Evaluation of the enzyme-linked immunosorbent assay in the detection of cattle infected with Brucella abortus. Vet Microbiol. (1984) 10 (1): 23-32. Sutherland S.S.
  4. Serological follow-up of human brucellosis by measuring IgG antibodies to lipopolysaccharide and cytoplasmic proteins of Brucella species. Clin Infect Dis. (1996) 22 (3): 446-55. Baldi P.C., et al.
  5. [Serological diagnosis of brucellosis in cattle. A comparison between ELISA (IgG) and classical serological tests]. Dev Biol Stand. (1984) 56: 451-63. French. Trap D. and Gaumont R.

ADDITIONAL INFORMATION

  • Full Name: Brucella Abortus IgG ELISA Kit
  • Reactivity: Human
  • Sample Type: Serum, Plasma
  • Sensitivity: 1.11 U/ml

OTHER RELATED ELISA KITS

TESTIMONIALS arrow icon

Your secretory IgA ELISA kit gave good results and I was also really impressed with how quickly we received it.

L. Johnston
PhD Student / University of Glasgow

It is refreshing to know that you have a technical team that is very knowledgeable. I have already recommended your company to other researchers in our department.

Dr. P. Anderson
Lecturer / University College London (UCL)

I am a first time user and found that your instruction manual was very easy to follow. The insulin ELISA kit performed well and I was happy with the results that were generated.

J. Thomas
Senior Technician / Addenbrooke’s Hospital

I carried out a pilot study comparing the performance of many ELISA kits from different suppliers and found your kits to be one of the better performers. We observed good linearity and tight replicates.

Dr. C. Davies
Lead Scientists / AstraZeneca

You are my first point of contact when I am looking to purchase ELISA kits. You have such an easy and simple system, yet it is very effective.

A. Shaw
Purchasing / University of Oxford