Brucella Abortus IgG ELISA Kit

Full Name: Brucella Abortus IgG ELISA Kit
Reactivity: Human
Sample Type: Serum, Plasma
Sensitivity: 1.11 U/ml

BACKGROUND

There are several Brucella species that infect different animal reservoirs, but all share similar biology. Brucella has a cell wall structure containing lipopolysaccharide that facilitates evasion of phagocytosis. Its cell membrane possesses phospholipids and outer membrane proteins involved in virulence.

A key aspect of Brucella pathogenesis is its ability to invade and survive within macrophages and other phagocytic cells, replicating in a membrane-bound compartment. Virulence factors include surface proteins that mediate adhesion to host cells. Brucella also utilizes a type IV secretion system to inject effector proteins into host cells that hijack their functions to promote bacterial survival and replication. Additionally, Brucella secretes factors that inhibit macrophage activation and prevent apoptosis.

In animals, Brucella causes reproductive diseases like abortions and sterility. Zoonotic transmission to humans occurs through ingestion of infected foods or contact with tissues and secretions from infected animals. Human brucellosis typically manifests with undulant fever, arthralgia, and malaise, but can also affect organs throughout the body. Chronic brucellosis is difficult to cure with antibiotics because cells harboring dormant Brucella are not eliminated.

INTENDED USE

Human Brucella abortus IgG ELISA kit is a procedure intended for detecting in-vitro concentrations of human IgG antibodies against Brucella abortus (Brucella IgG) in plasma or serum. This assay has a minimum analytical sensitivity limit of 1.11 U/ml.

CONTENT

All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.

• Brucella antigen coated microtiter strips.
• Enzyme Conjugate.
• Sample Diluent.
• TMB Substrate.
• Calibrator D (Positive Control).
• Calibrator C (Weak Positive Control).
• Calibrator A (Negative Control).
• Calibrator B (Cut-Off Standard).
• Washing Buffer (10x).
• Stop Solution.

SENSITIVITY

The minimum detection sensitivity level of human IgG antibodies to Brucella abortus (Brucella-IgG) using current Brucella abortus IgG ELISA kit was 1.11 U/ml. The dynamic range for this assay is 1.0 – 150.0 U/ml.

ASSAY CHARACTERSTICS

– Intra-Assay-Precision: 8.7 %
– Inter-Assay-Precision: 7.9 %
– Inter-Lot-Precision: 4.1 – 8.8 %
– Analytical Sensitivity: 1.11 U/mL
– Recovery: 82 – 96 %
– Linearity: 80 – 115 %
– Cross-Reactivity: No cross-reactivity to Bordetella pertussis
– Interferences: No interferences to bilirubin up to 0.3 mg/mL, hemoglobin up to 8.0 mg/mL and triglycerides up to 5.0 mg/mL
– Clinical Specificity: 100 %
– Clinical Sensitivity: 100 %

REFERENCES

1. Immunoglobulin G binding activity of Brucella abortus. Mol Immunol. (1991) 28 (1-2): 35-9. Bricker B.J., et al.
2. [Serological diagnosis of brucellosis in cattle. A comparison between ELISA (IgG) and classical serological tests]. Dev Biol Stand. (1984) 56: 451-63. French. Trap D. and Gaumont R.
3. Evaluation of indirect enzyme-linked immunosorbent assays and IgG avidity assays using a protein A-peroxidase conjugate for serological distinction between Brucella abortus S19-vaccinated and -infected cows. Clin Vaccine Immunol. (2010) 17 (4): 588-95. Pajuaba A.C., et al.
4. Serological follow-up of human brucellosis by measuring IgG antibodies to lipopolysaccharide and cytoplasmic proteins of Brucella species. Clin Infect Dis. (1996) 22 (3): 446-55. Baldi P.C., et al.
5. Evaluation of the enzyme-linked immunosorbent assay in the detection of cattle infected with Brucella-abortus. Vet Microbiol. (1984) 10 (1): 23-32. Sutherland S.S.

OTHER RELATED ELISA KITS

• Brucella IgM ELISA Kit
• Borrelia IgG ELISA Kit
• Borrelia IgM ELISA Kit