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Brazil Nut ELISA Kit

  • Created on the 3 October, 2017.

BACKGROUND

Brazil nut (Bertholletia excelsa) belongs to the Lecythidaceae family and is the only species in the montypic genus Bertholletia. It is often found in South America and is native to countries such as Brazil, Colombia, Bolivia, Venezuela, Peru and Guianas. This family is in the order Ericales, which includes some other more well-known plants for example cranberries, blueberries, tea, sapote, phlox, persimmons and gutta-percha. Brazil nut allergies are usually more commonly found in adults rather than children, allergic reactions are frequently severe and also have the potential to cause anaphylaxis (such as breathing difficulty). Brazil nuts can be eaten raw and are often used in cakes, biscuits, bread, muesli, ice cream and confectionery. Also, people who are allergic to Brazil nuts have a unique problem becuase it is the only allergic reaction which can be sexually transmissible (i.e. the semen of a man who has eaten brazil nuts is able to trigger an allergic response if his sexual partner has an allergy). The following ELISA assay offers a highly sensitive detection system for the quantification of residues in sausage, cookies, chocolate, ice-cream and cereals.

INTENDED USE

Brazil nut ELISA kit is a method for detecting quantitative amounts of Brazil nut in food (such as ice-cream, cookies, chocolate, cereals and sausage). This assay has a minimum analytical sensitivity limit of 0.02 ppm.

SENSITIVITY

The minimum detection sensitivity level of Brazil nut using this Brazil nut ELISA kit was 0.02 ppm. The standard assay range for this kit is 1.0 – 40.0 ppm.

REFERENCES

  1. A sensitive and robust competitive enzyme-linked immunosorbent assay for Brazil nut (Bertholletia excelsa L.) detection. J Agric Food Chem. (2009) 57 (2): 769-76. Sharma G.M., et al.
  2. Immunological analysis of allergenic cross-reactivity between peanut and tree nuts. Clin Exp Allergy. (2003) 33 (9): 1273-80. de Leon M.P., et al.
  3. Selection of recombinant antibodies by phage display technology and application for detection of allergenic Brazil nut (Bertholletia excelsa) in processed foods. J Agric Food Chem. (2013) 61 (43): 10310-9. de la Cruz S., et al.
  4. Multi-allergen screening immunoassay for the detection of protein markers of peanut and four tree nuts in chocolate. Food Addit Contam. (2005) 22 (8): 709-15. Ben Rejeb S., et al.
  5. Allergy to minor allergens of Brazil nut. Allergy. (2002) 57 (11): 1080-1. Asero R., et al.

ADDITIONAL INFORMATION

  • Full Name: Βrazil Nut ELISA Kit
  • Sample Type: Food
  • Sensitivity: 0.02 ppm

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