Brazil Nut ELISA Kit

  • Created on the 3 October, 2017.


Brazil nut ELISA kit is a method for detecting quantitative amounts of Brazil nut in food (such as ice-cream, cookies, chocolate, cereals and sausage). This assay has a minimum analytical sensitivity limit of 0.02 ppm.


Brazil nut (Bertholletia excelsa) belongs to the Lecythidaceae family and is the only species in the montypic genus Bertholletia. It is often found in South America and is native to countries such as Brazil, Colombia, Bolivia, Venezuela, Peru and Guianas. This family is in the order Ericales, which includes some other more well-known plants for example cranberries, blueberries, tea, sapote, phlox, persimmons and gutta-percha. Brazil nut allergies are usually more commonly found in adults rather than children, allergic reactions are frequently severe and also have the potential to cause anaphylaxis (such as breathing difficulty). Brazil nuts can be eaten raw and are often used in cakes, biscuits, bread, muesli, ice cream and confectionery. Also, people who are allergic to Brazil nuts have a unique problem becuase it is the only allergic reaction which can be sexually transmissible (i.e. the semen of a man who has eaten brazil nuts is able to trigger an allergic response if his sexual partner has an allergy). The following Brazil nut ELISA kit offers a highly sensitive detection system for the quantification of residues in sausage, cookies, chocolate, ice-cream and cereals.


The minimum detection sensitivity level of Brazil nut using current Brazil nut ELISA kit was 0.02 ppm. The standard range for this assay is 1.0 – 40.0 ppm.


  1. A sensitive and robust competitive enzyme-linked immunosorbent assay for Brazil nut (Bertholletia excelsa L.) detection. J Agric Food Chem. (2009) 57 (2): 769-76. Sharma G.M., et al.
  2. Immunological analysis of allergenic cross-reactivity between peanut and tree nuts. Clin Exp Allergy. (2003) 33 (9): 1273-80. de Leon M.P., et al.
  3. Selection of recombinant antibodies by phage display technology and application for detection of allergenic Brazil nut (Bertholletia excelsa) in processed foods. J Agric Food Chem. (2013) 61 (43): 10310-9. de la Cruz S., et al.
  4. Multi-allergen screening immunoassay for the detection of protein markers of peanut and four tree nuts in chocolate. Food Addit Contam. (2005) 22 (8): 709-15. Ben Rejeb S., et al.
  5. Allergy to minor allergens of Brazil nut. Allergy. (2002) 57 (11): 1080-1. Asero R., et al.


  • Full Name: Βrazil Nut ELISA Kit
  • Sample Type: Food
  • Sensitivity: 0.02 ppm



Your secretory IgA ELISA kit gave good results and I was also really impressed with how quickly we received it.

L. Johnston
PhD Student / University of Glasgow

It is refreshing to know that you have a technical team that is very knowledgeable. I have already recommended your company to other researchers in our department.

Dr. P. Anderson
Lecturer / University College London (UCL)

I am a first time user and found that your instruction manual was very easy to follow. The insulin ELISA kit performed well and I was happy with the results that were generated.

J. Thomas
Senior Technician / Addenbrooke’s Hospital

I carried out a pilot study comparing the performance of many ELISA kits from different suppliers and found your kits to be one of the better performers. We observed good linearity and tight replicates.

Dr. C. Davies
Lead Scientists / AstraZeneca

You are my first point of contact when I am looking to purchase ELISA kits. You have such an easy and simple system, yet it is very effective.

A. Shaw
Purchasing / University of Oxford