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Brain Natriuretic Peptide (BNP) ELISA Kit

  • Created on the 16 May, 2017.

BACKGROUND

Brain natriuretic peptide which can also be called B-type natriuretic peptide (BNP) or natriuretic peptide B or ventricular natriuretic peptide, it is secreted in response to excessive stretching of the heart muscle cells by the ventricles. BNP is secreted attached to a prohormone (NT-proBNP), this is a 76 amino acid N-terminal fragment and it is biologically inactive. Once it is released BNP will bind and activate the atrial natriuretic factor receptors (NPRA) and the physiological actions include decreasing central venous pressure and systemic vascular resistance, in addition to an increase in natriuresis. The overall action of both BNP leads to a decrease in cardiac output, which ultimately results in a reduction in blood volume that follows diuresis and natriuresis. Both BNP and NT-proBNP have been useful in screening and prognosis of heart failure. The levels of both of these is typically elevated in patients having left ventricular dysfunction. Also, recombinant BNP and nesiritide have been vital in treating decompensate heart failure.

INTENDED USE

Human BNP ELISA kit is designed for analysing in vitro quantitative levels of brain natriuretic peptide in human serum, plasma, other biological fluids, cell culture supernatant and cell lysates. This assay has a minimum sensitivity limit of 2.56 ng/L.

SENSITIVITY

The minimum detection sensitivity level of human BNP (B-type natriuretic peptide) using this human brain natriuretic peptide ELISA kit was 2.56 ng/mL. The dynamic assay range for this kit is 5.0 – 2,000.0 ng/L.

REFERENCES

  1. Brain-natriuretic peptide: Much more than a biomarker. Int J Cardiol. (2016) 221: 1031-8. Review. Calzetta L., et al.
  2. BNP and NT-proBNP elevation after running–a systematic review. Acta Cardiol. (2015) 70 (5): 501-9. Review. Vilela E.M., et al.
  3. B-type natriuretic peptide-guided therapy: a systematic review. Heart Fail Rev. (2014) 19 (4): 553-64. Review. Balion C., et al.
  4. The potential and limitations of plasma BNP measurement in the diagnosis, prognosis, and management of children with heart failure due to congenital cardiac disease: an update. Heart Fail Rev. (2014) 19 (6): 727-42. Review. Cantinotti M., et al.
  5. State of the art of immunoassay methods for B-type natriuretic peptides: An update. Crit Rev Clin Lab Sci. (2015) 52 (2): 56-69. Review. Clerico A., et al.

ADDITIONAL INFORMATION

  • Full Name: Brain Natriuretic Peptide (BNP) ELISA Kit
  • Reactivity: Human
  • Sample Type: Serum, Cell Culture Supernatant, Plasma, Cell Lysates, Other Biological Fluids
  • Sensitivity: 2.56 ng/L

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