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Bone Morphogenetic Protein-4 (BMP-4) ELISA Kit

  • Created on the 6 February, 2017.

BACKGROUND

Bone morphogenetic protein 4 (BMP-4) in humans is encoded by the BMP4 gene that is located on 14q22-q23.1, it is a polypeptide protein which is part of the transforming growth factor-beta superfamily. Some of its important functions include: bone and cartilage development, in particular specifically limp and tooth development and repair of fractures. BMP4 is a critical signalling molecule in human embryonic development where it is required for early differentiation of the embryo and in establishing the dorsal-ventral axis. It is known to be secreted from the dorsal portion of the notochord and can act in concert with sonic hedgehog to establish a dorsal-ventral axis that can be used for the differentiation of later structures.

Other roles included bone mineralization, muscle and uteric bud development, it has also been implicated in fibrodysplasia ossificans progressive where it is found to be under expressed. BMP4 can stimulate the differentiation of overlying ectodermal tissue whilst the inhibition of the BMP4 signal by chordin, follistatin or noggin is known to causes the ectoderm to differentiate into the neural plate. This ELISA assay uses a standard that is recombinant BMP-4 that has a molecular mass of 26KDa.

INTENDED USE

Human BMP-4 ELISA kit is a reliable quantitative procedure for measuring bone morphogenetic protein-4 (BMP-4) in human bone tissue and cell culture supernatant. This assay has a minimum sensitivity detection limit of < 4.0 pg/ml.

SENSITIVITY

The minimum sensitivity detection limit of bone morphogenetic protein-4 (BMP-4) using this human BMP-4 ELISA kit was approximately < 4.0 pg/ml. The dynamic assay range for this kit is 62.5 – 4000.0 pg/ml.

REFERENCES

  1. Pituitary tumors: cell type-specific roles for BMP-4. Mol Cell Endocrinol. (2010) 326 (1-2): 85-8. Review. Labeur M., et al.
  2. CCN3 increases BMP-4 expression and bone mineralization in osteoblasts. J Cell Physiol. (2012) 227 (6): 2531-41. Tan T.W., et al.
  3. Fibroregulation of mesenchymal progenitor cells by BMP-4 after traumatic muscle injury. J Orthop Trauma. (2012) 26 (12): 693-8. Kluk M.W., et al.
  4. A role for bone morphogenetic protein-4 in lymph node vascular remodeling and primary tumor growth. Cancer Res. (2011) 71 (20): 6547-57. Farnsworth R.H., et al.

ADDITIONAL INFORMATION

  • Full Name: Bone Morphogenetic Protein-4 (BMP-4) ELISA Kit
  • Reactivity: Human
  • Sample Type: Bone Tissue, Cell Culture Supernatant
  • Sensitivity: < 4.0 pg/ml

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