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Beta-Lactoglobulin ELISA Kit

  • Created on the 3 October, 2017.

BACKGROUND

Βeta-Lactoglobulin (beta-LG) is a protein which is present in majority of ruminant species and is found in the milk of many other species. It is main whey protein found in cow and sheep’s milk (approx. 3g/l), belonging to the lipocalins family that has the ability to bind a large number of hydrophobic molecules (indicating a potential transport function). Lipocalins proteins also contain molecular pockets that allow them to accommodate iron complexes (siderohores). b-lactoglobulin accounts for a significant proportion of cow’s milk allergy, especially those found in children. Very low amounts of bovine milk is found to cause an allergic reaction, in severe cases this can lead to anaphylactic shock. Also, the presence of hidden milk proteins for example in cookies, convenience food, sausage and beverages may cause critical problems to individuals that are allergic to milk. This ELISA assay offers a highly sensitive detection system which can be used for identifying and quantifying bovine milk residues in cereals, cookies, chocolate, orange juice, sausage, soy products and wine.

INTENDED USE

Βeta lactoglobulin ELISA kit can be used to measuring quantitative amounts of ß-lactoglobulin (beta-LG) in food samples (for example chocolate, cookies, cereals, soy products, sausage, wine and orange juice. This assay has a minimum analytical sensitivity limit of 1.5 ppm.

SENSITIVITY

The minimum detection sensitivity level of ß-lactoglobulin (beta-LG) using this beta lactoglobulin ELISA kit was 1.5 ppm. The standard assay range for this kit is 10.0 – 400.0 ppm.

REFERENCES

  1. Comparison of immunomodulating properties of Beta-lactoglobulin and its hydrolysates. Iran J AllergyAsthma Immunol. (2014) 13 (1): 26-32. Duan C.C., et al.
  2. ELISA testing for soy antigens in dry dog foods used in dietary elimination trials. J Am Anim Hosp Assoc. (2014) 50 (6): 383-9. Willis-Mahn C., et al.
  3. A comparison of commercially available peanut ELISA test kits on the analysis of samples of dark and milk chocolate. J Immunoassay Immunochem. (2002) 23 (4): 451-9. Hurst W.J., et al.
  4. Food allergy: a winding road to the present. Pediatr AllergyImmunol. (2014) 25 (1): 25-6. Sampson H.A.
  5. Yogurt in the Treatment of β-Lactoglobulin-Induced Gastrointestinal Cow’s Milk Allergy. J Investig Allergol Clin Immunol. (2016) 26 (5): 327-329. Poza-Guedes P., et al.
  6. T-cell epitope-containing hypoallergenic β-lactoglobulin for oral immunotherapy in milk allergy. Pediatr AllergyImmunol. (2016) 27 (8): 818-824. Ueno H.M., et al.

ADDITIONAL INFORMATION

  • Full Name: Βeta-Lactoglobulin ELISA Kit
  • Sample Type: Food
  • Sensitivity: 1.5 ppm

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Your secretory IgA ELISA kit gave good results and I was also really impressed with how quickly we received it.

L. Johnston
PhD Student / University of Glasgow

It is refreshing to know that you have a technical team that is very knowledgeable. I have already recommended your company to other researchers in our department.

Dr. P. Anderson
Lecturer / University College London (UCL)

I am a first time user and found that your instruction manual was very easy to follow. The insulin ELISA kit performed well and I was happy with the results that were generated.

J. Thomas
Senior Technician / Addenbrooke’s Hospital

I carried out a pilot study comparing the performance of many ELISA kits from different suppliers and found your kits to be one of the better performers. We observed good linearity and tight replicates.

Dr. C. Davies
Lead Scientists / AstraZeneca

You are my first point of contact when I am looking to purchase ELISA kits. You have such an easy and simple system, yet it is very effective.

A. Shaw
Purchasing / University of Oxford