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Beta-Lactoglobulin ELISA Kit

INTENDED USE

Beta-lactoglobulin ELISA kit can be used to measuring quantitative amounts of ß-lactoglobulin (beta lactoglobulin, beta-LG, beta-lactoglobulin) in food samples (for example chocolate, cookies, cereals, soy products, sausage, wine and orange juice). This assay has a minimum analytical sensitivity limit of 1.5 ppm.

BACKGROUND

Beta-Lactoglobulin (beta-LG) is a protein which is present in majority of ruminant species and is found in the milk of many other species. It is main whey protein found in cow and sheep’s milk (approx. 3g/l), belonging to the lipocalins family that has the ability to bind a large number of hydrophobic molecules (indicating a potential transport function). Lipocalins proteins also contain molecular pockets that allow them to accommodate iron complexes (siderohores).

Beta-lactoglobulin accounts for a significant proportion of cow’s milk allergy, especially those found in children. Very low amounts of bovine milk is found to cause an allergic reaction, in severe cases this can lead to anaphylactic shock. Also, the presence of hidden milk proteins for example in cookies, convenience food, sausage and beverages may cause critical problems to individuals that are allergic to milk.

BETA-LACTOGLOBULIN ELISA KIT CONTETNT

All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.

  • Microtiter Plate: Coated with anti-ß-lacto-globulin antibodies.
  • Beta-Lactoglobulin Standards 1-5: Concentration 0, 10, 40, 100, 400 ppb.
  • Conjugate (anti-ß-lactoglobulin-peroxidase).
  • Substrate Solution (TMB).
  • Stop Solution (0.5 M H2SO4).
  • Extraction and sample dilution buffer (Tris)(10x).
  • Washing Solution (10x Concentrate).
  • Instruction Manual.

SENSITIVITY

The minimum detection sensitivity level of ß-lactoglobulin (beta-LG, beta lactoglobulin) using current beta-lactoglobulin ELISA kit was 1.5 ppm. The standard range for this assay is 10.0 – 400.0 ppm.

ASSAY CHARACTERISTICS

– Beta-Lactoglobulin: ELISA
– Sensitivity: Limit of detection, LOD (1.5ppm), Limit of quantification, LOQ (10ppm)
– Cross Reactivity: The following displayed some cross reactions: Ewe’s milk (< 0.2%), Casein (< 0.02%), Goat’s milk (< 0.002%). No cross reaction was detected for the following: Egg, Poppy seed, Walnut, Chick pea, Peach, Wheat, Sesame, Pecan nut, Pea, Apricot, Oats, Sunflower seed, Brazil nut, Bean, Cherry, Rye, Pumpkin seed, Coconut, Lecithin, Plum, Barley, Pine nut, Almond, Saccharose, Beef, Rice, Cashew nut, Pistachio, Cocoa, Pork, Corn, Peanut, Macadamia, Orange, Chicken, Buckwheat, Hazelnut, Chestnut, Wine, Bovine albumin, Soy.
– Intra-Assay Precision: 7%
– Inter-Assay Precision: 9 – 12%
– Linearity: 72 – 127%
– Recovery: Sausage (107%), Orange juice (98%), Cereals (94%), Cookies (88%), Chocolate (86%), White wine (82%), Soy milk (70%).

REFERENCES

  1. Comparison of immunomodulating properties of Beta-lactoglobulin and its hydrolysates. Iran J AllergyAsthma Immunol. (2014) 13 (1): 26-32. Duan C.C., et al.
  2. ELISA testing for soy antigens in dry dog foods used in dietary elimination trials. J Am Anim Hosp Assoc. (2014) 50 (6): 383-9. Willis-Mahn C., et al.
  3. A comparison of commercially available peanut ELISA test kits on the analysis of samples of dark and milk chocolate. J Immunoassay Immunochem. (2002) 23 (4): 451-9. Hurst W.J., et al.
  4. Food allergy: a winding road to the present. Pediatr AllergyImmunol. (2014) 25 (1): 25-6. Sampson H.A.
  5. Yogurt in the Treatment of β-Lactoglobulin-Induced Gastrointestinal Cow’s Milk Allergy. J Investig Allergol Clin Immunol. (2016) 26 (5): 327-329. Poza-Guedes P., et al.
  6. T-cell epitope-containing hypoallergenic β-lactoglobulin for oral immunotherapy in milk allergy. Pediatr AllergyImmunol. (2016) 27 (8): 818-824. Ueno H.M., et al.

ADDITIONAL INFORMATION

  • Full Name: Beta-Lactoglobulin ELISA Kit
  • Sample Type: Food (Soy Products, Chocolate, Cookies, Sausage, Cereals, Orange Juice, Wine)
  • Sensitivity: 1.5 ppm

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