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Beta-2 Microglobulin (B2M) ELISA Kit

INTENDED USE

Human beta-2 microglobulin ELISA kit is designed to analyse in vitro quantitative concentrations of ß2-microglobulin (B2M, beta-2 microglobulin) in human serum, plasma and urine samples. This assay has a minimum sensitivity limit of 0.1 µg/ml.

BACKGROUND

Beta-2 microglobulin (also known as B2M) is a small membrane protein which is associated to the heavy chains of MHC class 1 and therefore can be found on the surfaces of all nucleated cells (except red blood cells). Due to the fact that they are small, this allows them to pass through the glomerular membrane but large quantities of the protein are almost completely reabsorbed by the proximal tubules. Serum levels of beta-2 microglobulin are found to be elevated in diseases which are linked with an increase in cell turnover. There are also evidence indicating elevated levels present in several benign conditions for example renal dysfunction, liver disease, chronic inflammation, some acute viral infections and a number of malignancies.

B2M is a useful tumour marker for a number of blood cell cancers. It is not intended for the diagnostic of a specific disease, but instead it is associated with the amount of cancer that is present (i.e. tumour burden) and more importantly can be used to provide further information about patient prognosis. It is also often used to help to determine the severity and spread (stage) of multiple myeloma and in some cases provide an aid to evaluate the disease activity and the effectiveness of any treatment given. The measurement of B2M in plasma and serum samples can aid in the clinical assessment of the activation of a tumor marker and the cellular immune system. The urine values indicate a number of renal filtration disorders, since the molecule is not stable in urine for a long time due the acid pH-values. ß2-microglobulin is synthesized in the lymphatic system and its measurement in serum and urine can determine the differentiation between a disturbance of the kidney function and an activation of the lymphatic system.

BETA-2 MICROGLOBULIN ELISA KIT CONTENT

All reagents supplied need to be stored at 2 °C – 8 °C in the dark. Do not expose reagents to heat, sun, or strong light during storage and usage.

  • One Divisible Microplate: Coated with highly purified anti-human-β2-microglobulin IgG (rabbit, polyclonal).
  • Calibrator A-F: Concentration 0, 0.75, 1.5, 3, 6, 12 µg/ml.
  • Positive Control.
  • Negative Control.
  • Sample Buffer.
  • Enzyme Conjugate containing anti-human ß2-microglobulin antibodies.
  • TMB Substrate.
  • Stop Solution.
  • Wash Buffer (50x Conc).

SENSITIVITY

The minimum detection sensitivity level of human beta-2-microglobulin (ß2-microglobulin, B2M) using current beta-2 microglobulin ELISA kit was 0.1 µg/ml. The dynamic range for this assay is 0.75 – 12.0 µg/ml.

ASSAY CHARACTERISTICS

– Beta 2 Microglobulin (B2M): ELISA
– Intra-Assay Variation: 2.6 – 4.2%
– Inter-Assay Variation: 3.8 – 4.9%
– Sensitivity: 0.1 μg/mL
– Specificity: Highly specific for human β2-microglobulin. No cross reactivities have been observed.
– Interfering Substances: No interference has been observed with haemolytic (up to 1000 mg/dL), lipemic (up to 3 g/dL triglycerides) or bilirubin (up to 40 mg/dL) containing sera.

REFERENCES

  1. The Implication and Significance of Beta 2 Microglobulin: A Conservative Multifunctional Regulator. Chin Med J (Engl). (2016) 129 (4): 448-55. Review. Li L., et al.
  2. Beta(2)-microglobulin removal by extracorporeal renal replacement therapies. Biochim Biophys Acta. (2005) 1753 (1): 146-53. Review. Krieter D.H., et al.
  3. β2-Microglobulin-mediated signaling as a target for cancer therapy. Anticancer Agents Med Chem. (2014) 14 (3): 343-52. Review. Nomura T., et al.
  4. beta 2-Microglobulin: structure, function and significance. Vox Sang. (1980) 38 (6): 323-7. Review. Bernier G.M.
  5. Killing tumor cells through their surface beta(2)-microglobulin or major histocompatibility complex class I molecules. Cancer. (2010) 116 (7): 1638-45. Review. Yang J. and Yi Q.

ADDITIONAL INFORMATION

  • Full Name: Beta-2 Microglobulin (B2M) ELISA Kit
  • Reactivity: Human
  • Sample Type: Serum, Urine, Plasma
  • Sensitivity: 0.1 µg/ml

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