BPI ELISA Kit (Bactericidal/Permeability-Increasing Protein)

INTENDED USE

Human BPI ELISA kit can be used for measuring in vitro quantitative concentrations of IgG autoantibodies to bactericidal/permeability increasing protein (BPI IgG, BPI) in human seruma and plasma. This assay has a minimum analytical sensitivity limit of 0.5 U/ml.

BACKGROUND

Bactericidal/permeability increasing protein (BPI) is a 456 residue cationic protein which is approx. 50kDa in size, produced only by precursors of polymorphonuclear leukocytes (PMN) and forms part of the innate immune system. It belongs to a larger group of proteins which are more commonly known as lipid transfer (LT)/LBP family, members of this family possess the ability to bind or attach to many different types of lipid substrates.

BPI has a potent killing activity against gram-negative bacteria and is often referred to as an endogenous antibiotic protein. It binds to compounds which are known as lipopolysaccharides, these are potent activators of the immune system. There is also evidence to suggest that a certain concentration of it can prevent this activation process. Diseases that are linked with it can often be found to contain mutations of BPI or the presence of the antineutrophil cytoplasmic Ab (ANCA) directed against BPI.

CONTENT

All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.

• Human BPI Coated Microplate (Dismountable).
• Lyophilized Standard: Concentrations 0, 6.3, 12.5, 25, 50, 100.0 U/ml.
• Positive And Negative Controls.
• Sample/Standard Dilution Buffer.
• Biotin-labelled Antibody (Concentrated).
• Antibody Dilution Buffer.
• HRP-Streptavidin Conjugate (SABC).
• SABC Dilution Buffer.
• TMB Substrate.
• Stop Solution.
• Wash Buffer (25x).

SENSITIVITY

The minimum detection sensitivity level of IgG antibodies to bactericidal/permeability increasing protein (BPI, BPI IgG) using current BPI ELISA kit was 0.5 U/ml. The dynamic range for this assay is 6.3 – 100.0 U/ml.

ASSAY CHARACTERISTICS

– Results Interpretation: BPI Ab IgG serum samples from healthy blood donors display normal: < 10 U/ml, elevated: ³ 10 U/ml.
– Sensitivity: 0.5 U/ml
– Specificity: Specific for autoantibodies directed against BPI Ab, no cross crossreactivities to the other ANCA antigens have been observed.
– Interfering Substances: None

REFERENCES

1. Meet the relatives: a family of BPI- and LBP-related proteins. Trends Immunol. (2004) 25 (2): 53-5. Review. Bingle C.D. and Craven C.J.
2. Prospects for use of recombinant BPI in the treatment of gram-negative bacterial infections. Infect Agents Dis. (1995) 4 (2): 102-9. Review. Elsbach P. and Weiss J.
3. Antimicrobial peptides and colitis. Curr Pharm Des. (2013) 19 (1): 40-7. Review. Ho S., et al.
4. Expression of BPI (bactericidal/permeability-increasing-protein) in human mucosal epithelia. Biochem Soc Trans. (2003) 31 (Pt 4): 795-800. Review. Levy O., et al.
5. Antimicrobial peptides: the ancient arm of the human immune system. Virulence. (2010) 1 (5): 440-64. Review. Wiesner J. and Vilcinskas A.

ADDITIONAL INFORMATION

• Full Name: BPI ELISA Kit (Bactericidal/Permeability-Increasing Protein)
• Reactivity: Human
• Sample Type: Serum, Plasma
• Sensitivity: 0.5 U/ml

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