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B-Cell Lymphoma 2 (Bcl-2) ELISA Kit

INTENDED USE

Human Bcl-2 ELISA kit is a procedure intended for measuring in vitro quantitative amounts of B-cell lymphoma 2 (Bcl-2) in human cell culture supernatant, plasma, serum, cell lysates, tissue homogenates and many other biological fluids. This assay has a minimum sensitivity limit of < 1.15 U/ml.

BACKGROUND

B-cell lymphoma 2 (Bcl-2) belongs to the BCL-2 family of regulatory proteins which are known to be vital in regulating apoptosis by either inhibiting (anti-apoptotic) or by inducing (pro-apoptotic) process. It has been specifically referred to as crucial anti-apoptotic protein and but not a proto-oncogene since it is unable to transduce growth signals. Bcl-2 has the ability to initiate a new gene family that is involved in the regulation of cell death and survival without affecting cell proliferation. This type of precise regulation and maintenance of balance between cell death and cell proliferation in multicellular organisms is very important for tissue homeostasis.

The gene is encoded by a proto-oncogene and it is described as an intracellular membrane associated protein which essentially functions to block programmed cell death. The expression of Bcl-2 has been identified in a number of different epithelial malignancies, hematopoietic cells and non-neoplastic epithelia. The levels of Bcl-2 have been found to be low or even undetectable in endothelial cells. The B-cell lymphoma 2 gene is known to have a unique function among many mammalian oncogenes since it is involved in the negative regulation of apoptosis, where a decrease in its levels can lead to cell death by apoptosis.

On the other hand, overexpression of Bcl-2 is able to protect cells from death, however, this is neither able to lead to tumorgenic transformation of immortalized cells or immortalize normal cells. There have a many homologs of Bcl-2 which have been discovered, some of them are able to function as inhibitors of cell death whereas others can promote the process of apoptosis (i.e. are opposing the function of Bcl-2). Damage occurring to the Bcl-2 gene can lead a number of cancers such as chronic lymphocytic leukemia, melanoma, lung, breast and prostate cancer, it has also been identified as a possible cause for autoimmunity, schizophrenia and resistance to cancer treatments.

BCL-2 ELISA KIT CONTENT

All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.

  • One 96-Well Plate: Pre-coated with anti Bcl-2 antibody.
  • Standards: Concentrations 0, 1.562, 3.125, 6.25, 12.5, 25, 50, 100 ng/ml, made up from lyophilized recombinant human Bcl-2.
  • Sample/Standard Dilution Buffer.
  • Biotinylated-labelled Bcl-2 Antibody.
  • Antibody Dilution Buffer.
  • HRP-Streptavidin Conjugate (SABC).
  • SABC Dilution Buffer.
  • TMB Substrate.
  • Wash Buffer (25x).
  • Plate Sealer.
  • Product Instructions.

SENSITIVITY

The minimum detection sensitivity level of a B-cell lymphoma 2 (B-cell leukemia) using this human Bcl-2 ELISA kit was 0.938 ng/ml. The dynamic range for this assay is 1.0 – 700.0 U/ml.

ASSAY CHARACTERISTICS

– B-Cell Lymphoma 2 (Bcl-2): ELISA
– Specificity: Highly specific for Bcl-2, no cross reactivity or interference between Bcl-2 and analogues was detected.
– Recovery: Serum (85 – 101%), EDTA Plasma (86 – 105%), Heparin Plasma (87 – 103%).
– Linearity: Serum (92 – 104%), EDTA Plasma (82 – 99%), Heparin Plasma (83 – 97%).
– Precison Intra-Assay: CV < 8%.
– Precison Inter-Assay: CV < 10%.
– Stability: Less than 10%.

REFERENCES

  1. Discoveries and controversies in BCL-2 protein-mediated apoptosis. FEBS J. (2016) 283 (14): 2690-700. Review. Zheng J.H., et al.
  2. Targeting the B-cell lymphoma/leukemia 2 family in cancer. J Clin Oncol. (2012) 30 (25): 3127-35. Review. Davids M.S. and Letai A.
  3. Cell death regulation by B-cell lymphoma protein. Apoptosis. (2006) 11 (4): 459-71. Review. Verma Y.K., et al.
  4. Hematologic malignancies: newer strategies to counter the BCL-2 protein. J Cancer Res Clin Oncol. (2016) 142 (9): 2013-22. Review. Ebrahim A.S., et al.

ADDITIONAL INFORMATION

  • Full Name: B-Cell Lymphoma 2 (Bcl-2) ELISA Kit
  • Reactivity: Human
  • Sample Type: Plasma, Biological Fluids, Serum, Tissue Homogenates
  • Sensitivity: 0.938 ng/ml

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