- Created on the 28 March, 2017.
Human BAFF ELISA kit is designed to be used for measuring in vitro quantitative concentrations of B-cell activating factor (BAFF) in human cell culture supernatant, serum and plasma. This assay has a minimum detection limit of < 2.0 pg/ml.
B-cell activating factor (BAFF) which is also known as APOL-related leukocyte expressed ligand (TALL-1), dendritic cell-derived TNF-like molecule (CD257 antigen) and B Lymphocyte Stimulator (BLyS), is part of the tumor necrosis factor ligand superfamily member 13B proteins. BAFF is a glycoprotein which is 285 amino acid long at it has the ability to undergo glycosylation at residue 124. It is usually expressed as a membrane-bound type II transmembrane protein that is found on a number of different cells such as dendritic cells, bone marrow stromal cells and monocytes.
BAFF is the natural ligand for three tumor necrosis factor receptors which are called BAFF-R (BR3), BCMA, and TACI and cyclophilin ligand interactor), all of these are displaying differing binding affinities for it. Signalling through BAFF-R or BCMA can be used to stimulate B lymphocytes to order to undergo proliferation and to counter apoptosis. Whereas, BCMA displays an intermediate binding phenotype and will work with APRIL and BAFF. BAFF is a cytokine which is a member of the tumor necrosis factor (TNF) ligand family and is a ligand for receptors TNFRSF13B/TACI, TNFRSF13C/BAFF-R and TNFRSF17/BCMA. It is essentially expressed in B cell lineage cells which can act as a potent B cell activators, it also displays vital roles in B cell proliferation and differentiation. Excessive level of BAFF have be identified to result in abnormally high antibody production which can cause rheumatoid arthritis, systemic lupus erythmatosis and a number of other autoimmune diseases. The standard that is used in the following BAFF ELISA kit is a recombinant soluble human BAFF (A134-L295) that has a molecular mass of 19.6KDa.
The minimum detection sensitivity level of B-cell activating factor using current human BAFF ELISA kit was 2.0 pg/ml. The dynamic range for this assay is 62.5 – 4,000 pg/ml.
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- Full Name: B-Cell Activating Factor (BAFF) ELISA Kit
- Reactivity: Human
- Sample Type: Serum, Cell Culture Supernatant, Plasma
- Sensitivity: < 2.0 pg/ml