BAFF ELISA Kit (B-Cell Activating Factor)

INTENDED USE

Human BAFF ELISA kit is designed to be used for measuring in vitro quantitative concentrations of B-cell activating factor (BAFF) tissue homogenate, serum, plasma and other biological fluids. This assay has a minimum detection limit of 37.5 pg/ml.

BACKGROUND

B-cell activating factor (BAFF) which is also known as APOL-related leukocyte expressed ligand (TALL-1), dendritic cell-derived TNF-like molecule (CD257 antigen) and B Lymphocyte Stimulator (BLyS), is part of the tumor necrosis factor ligand superfamily member 13B proteins. It is a glycoprotein which is 285 amino acid long at it has the ability to undergo glycosylation at residue 124. It is expressed as a membrane-bound type II transmembrane protein and it is found on a number of cells such as; dendritic, bone marrow stromal and monocytes.

BAFF is the natural ligand for three tumor necrosis factor receptors which are called BAFF-R (BR3), BCMA, and TACI and cyclophilin ligand interactor), all of these are displaying differing binding affinities for it. Signalling through BAFF-R or BCMA can be used to stimulate B lymphocytes to order to undergo proliferation and to counter apoptosis. Whereas, BCMA displays an intermediate binding phenotype and will work with APRIL and BAFF. It is essentially expressed in B cell lineage cells which can act as a potent B cell activators, it also displays vital roles in B cell proliferation and differentiation.

CONTENT

All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.

• One 96-Well Plate: Pre-coated with anti-BAFF antibody.
• Standards: Concentrations 0, 62.5, 125, 250, 500, 1000, 2000, 4000 pg/ml, made up from lyophilized recombinant.
• Sample/Standard Dilution Buffer.
• Biotinylated-Labelled Antibody.
• Antibody Dilution Buffer.
• HRP-Streptavidin Conjugate (SABC).
• SABC Dilution Buffer.
• TMB Substrate.
• Wash Buffer (25x).
• Plate Sealer.
• Product Instructions.

SENSITIVITY

The minimum detection sensitivity level of B-cell activating factor using this BAFF ELISA kit was 37.5 pg/ml. The dynamic range for this assay is 62.5 – 4,000 pg/ml.

ASSAY CHARACTERISTICS

– Specificity: Highly specific for BAFF, no cross reactivity or interference between BAFF and analogues was detected.
– Recovery: Serum (88 – 101%), EDTA Plasma (86 – 105%), Heparin Plasma (89 – 104%).
– Linearity: Serum (86 – 104%), EDTA Plasma (83 – 101%), Heparin Plasma (81 – 86%).
– Precison Intra-Assay: CV < 8%.
– Precison Inter-Assay: CV < 10%.
– Stability: Less than 10%.

REFERENCES

1. BAFF and the regulation of B cell survival. Immunol Lett. (2003) 88 (1): 57-62. Review. Schneider P. and Tschopp J.
2. Functional and clinical aspects of the B-cell-activating factor (BAFF): a narrative review. Scand J Immunol. (2011) 73 (1): 1-7. Review. Lied G.A. and Berstad A.
3. The BAFF/APRIL system: an important player in systemic rheumatic diseases. Curr Dir Autoimmun. (2005) 8: 243-65. Review. Mackay F., et al.
4. BAFF inhibition: a new class of drugs for the treatment of autoimmunity. Exp Cell Res. (2011) 317 (9): 1270-7. Review. Liu Z. and Davidson A.

ADDITIONAL INFORMATION

• Full Name: BAFF ELISA Kit (B-Cell Activating Factor)
• Reactivity: Human
• Sample Type: Biological Fluids, Serum, Tissue Homogenates, Plasma
• Sensitivity: 37.5 pg/ml

OTHER RELATED ELISA KITS

• APP ELISA Kit
• MPO ELISA Kit
• FAS ELISA Kit