Human ANP ELISA kit is a procedure intended for determining in vitro quantitative amounts of atrial natriuretic peptide (ANP, atrial natriuretic factor, ANF, atrial natriuretic hormone, ANH) in human serum, cell lysates, plasma, other biological fluids and cell culture supernatant. This assay has a minimum sensitivity limit of 4.62 pg/ml.
Atrial natriuretic peptide (ANP) can also be referred to as atrial natriuretic hormone (ANH), atrial natriuretic factor (ANF), atriopeptin, cardionatrine and cardiodilatin. It is a 28 amino acid polypeptide hormone which is secreted by heart muscle cells, a powerful vasodilator. Human ANP is responsible for reducing the blood pressure by regulating homeostatic control of the body water, fat, sodium and potassium levels. It is often released by muscles cells in the atria of the heart in response to high blood volume. It can be secreted in response to raised sodium concentration, stretching of the atrial wall, endothelin, increased sympathetic stimulation of β-adrenoceptors and exercise.
Atrial natriuretic peptide is able to bind to a specific set of receptors which are called ANP receptors, this results in reducing blood volume, systemic blood pressure and a reduction in cardiac output. Generally, the function of ANP is to counter the increase in the volume and pressure of blood that is caused via the renin-angiotensin system. This atrial natriuretic peptide ELISA kit is an ideal tool for measuring concentrations of ANP, ANF and ANH using various different types of sample types. ANP and many of its related peptides are important biomarkers for many cardiovascular diseases such as coronary artery disease, stroke, heart failure and myocardial infarction. Also, recombinant atrial natriuretic peptide has been approved in Japan to be used in order to treat patients experiencing heart failure.
ANP ELISA KIT CONTENT
All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.
- Microwell Plate: Antibody specific to ANP is pre-coated wells.
- Standards: Concentrations 12.35, 37.04, 111.11, 333.33, 1,000 pg/ml
- Standard Diluent Buffer.
- Wash Buffer (3x Concentrated).
- Diluent A.
- Diluent B.
- Stop Solution.
- TMB Substrate.
- Detection Reagent A (100x).
- Detection Reagent B (100x).
- Plate Sealer.
The minimum detection sensitivity level of human atrial natriuretic peptide (ANH, atrial natriuretic hormone, ANP, ANF, atrial natriuretic factor) using current ANP ELISA kit was 4.62 pg/ml. The dynamic range for this assay is 12.35 – 1,000.0 pg/ml.
– Atrial Natriuretic Peptide (ANP): ELISA
– Intra Assay Precision: < 10%
– Inter Assay Precision: < 12%
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- Novel natriuretic peptides: new compounds and new approaches. Curr Heart Fail Rep. (2011) 8 (1): 22-7. Review. Vogel M.W and Chen H.H.
- Biomarkers and heart disease. Eur Rev Med Pharmacol Sci. (2014) 18 (19): 2927-35. Review. Sun R.R., et al.
- Roles of atrial natriuretic peptide and its therapeutic use. J Cardiol. (2010) 56 (3): 262-70. Review. Saito Y.
- Natriuretic peptides in the pathophysiology of congestive heart failure. Curr Cardiol Rep. (2000) 2 (3): 198-205. Review. Chen H.H. and Burnett J.C.
- Full Name: Atrial Natriuretic Peptide (ANP) ELISA Kit
- Reactivity: Human
- Sample Type: Cell Lysates, Plasma, Cell Culture Supernatant, Serum, Other Biological Fluids
- Sensitivity: 4.62 pg/ml