Human ASCA IgG/IgA ELISA kit is intended for analysing in vitro quantitative levels of IgA and IgG anti-saccharomyces cerevisiae antibodies (ASCA IgG or IgA) using human plasma or serum samples. This assay has a minimum analytical sensitivity limit of 0.5 U/ml.
Anti-saccharomyces cerevisiae antibodies (ASCAs) are present in people who have inflammatory bowel disease (IBD) and are a useful tool which can aid in distinguishing between ulcerative colitis and Crohn disease, both of which are the two most common types of IBD. The tests that are carried out involve detecting two classes of ASCA in blood, notably IgA and IgG.
The following human ASCA IgG/IgA ELISA kit can be used for a variety of diagnostic applications. Studies have identified 7% positive IgA and 5% IgG class in ASCA in ulcerative colitis, in comparison to Crohn’s disease where a sensitivity of 60% for IgA and 75% for IgG class ASCA can be observed. ASCAs and pANCAs are two vital biomarkers which are used for colitis discrimination, where pANCA tend to recognize ulcerative colitis and ASCA recognizes Crohn’s disease more frequently. Diseases in which ASCA have been identified include Celiac disease, Crohn’s disease, Colitis and Behçet’s disease.
ASCA IGG/IGA ELISA KIT CONTENT
All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.
- Divisible Microplate: Consisting of 12 modules of 8 wells, highly purified mannan from Saccharomyces cerevisiae (ASCA) is bound to microwells.
- Calibrator A-F: Concentration 0-100 U/ml, made up from human ASCA antibodies.
- Control Positive and Control negative: Containing ASCA antibodies in a serum/buffer matrix.
- Sample Buffer (5x).
- Enzyme Conjugate: Contains anti-human IgG antibodies, HRP labelled.
- Enzyme Conjugate: Contains anti-human IgA antibodies, HRP labelled.
- TMB Substrate
- Wash Buffer (50x).
- Instruction for Use.
- Certificate of Analysis.
The minimum detection sensitivity level of human anti saccharomyces cerevisiae antibodies (ASCA IgG and IgA) using current human ASCA IgG/IgA ELISA kit was 0.5 U/ml. The dynamic range for this assay is 6.3 – 100.0 U/ml.
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- Biomarkers of inflammatory bowel disease. Dis Markers. (2014) 2014: 710915. Review. Fengming Y. and Jianbing W.
- Full Name: ASCA IgG/IgA ELISA Kit
- Reactivity: Human
- Sample Type: Serum, Plasma
- Sensitivity: 0.5 U/ml