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ASCA IgG/IgA ELISA Kit

  • Created on the 26 May, 2017.

BACKGROUND

Anti-saccharomyces cerevisiae antibodies (ASCAs) are present in people who have inflammatory bowel disease (IBD) and are a useful tool which can aid in distinguishing between ulcerative colitis and Crohn disease, both of which are the two most common types of IBD. The tests that are carried out involve detecting two classes of ASCA in blood, notably IgA and IgG. Studies have identified 7% positive IgA and 5% IgG class in ASCA in ulcerative colitis, in comparison to Crohn’s disease where a sensitivity of 60% for IgA and 75% for IgG class ASCA can be observed. ASCAs and pANCAs are two vital biomarkers which are used for colitis discrimination, where pANCA tend to recognize ulcerative colitis and ASCA recognizes Crohn’s disease more frequently. Diseases in which ASCA have been identified include Celiac disease, Crohn’s disease, Colitis and Behçet’s disease.

INTENDED USE

Human ASCA ELISA kit is intended for analysing in vitro quantitative levels of IgA and IgG anti-saccharomyces cerevisiae antibodies (ASCA) using human plasma or serum samples. This assay has a minimum analytical sensitivity limit of 0.5 U/ml.

SENSITIVITY

The minimum detection sensitivity level of human anti-saccharomyces cerevisiae antibodies using this human ASCA ELISA kit was 0.5 U/ml. The dynamic assay range for this kit is 6.3 – 100.0 U/ml.

REFERENCES

  1. ASCA, ANCA, ALCA and Many More: Are They Useful in the Diagnosis of Inflammatory Bowel Disease? Dig Dis. (2016) 34 (1-2): 90-7. Review. Zhou G., et al.
  2. IBD serological panels: facts and perspectives. Inflamm Bowel Dis. (2007) 13 (12): 1561-6. Review. Peyrin-Biroulet L., et al.
  3. Inflammatory bowel diseases: from pathogenesis to laboratory testing. Clin Chem Lab Med. (2014) 52 (4): 471-81. Review. Basso D., et al.
  4. Diagnostic approach to IBD. Hepatogastroenterology. (2000) 47 (31): 44-8. Review. Vermeire S., et al.
  5. Biomarkers of inflammatory bowel disease. Dis Markers. (2014) 2014: 710915. Review. Fengming Y. and Jianbing W.

ADDITIONAL INFORMATION

  • Full Name: ASCA IgG/IgA ELISA Kit
  • Reactivity: Human
  • Sample Type: Serum, Plasma
  • Sensitivity: 0.5 U/ml

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