Anti-Spermatozoa Antibody (ASA) Seminal ELISA Kit

  • Created on the 26 May, 2017.


Antibodies that are directed against spermatozoa antigens are found to cause infertility in both men and woman. Anti-sperm antibodies are known to fight against man’s sperm in the blood, semen or vaginal fluids. These can cause an immune system response which can either damage or even kill sperm in the bodies of men or women. There is evidence to indicate that if elevated levels of sperm antibodies come into contact with sperm they can make it very difficult or even impossible for the sperm to fertilise an egg (often referred to immunologic infertility). A women can produce sperm antibodies through an allergic reaction to the partner’s semen, whereas in men if the sperm comes into contact with his immune system this can lead to the production of sperm antibodies.  The use of anti-spermatozoa antibody ELISA assay is widely recommended in the diagnosis of immunologically caused disorders of fertility. This kit can also be applied for clinical practices to the diagnosis of infertility in men. Please note: the terms anti-sperm antibodies, anti-spermatozoa antibodies and sperm antibodies are equivalent.


Human anti-sperm antibody ELISA kit is designed for detecting in vitro quantitative levels of antibodies directed against human spermatozoa antigens using seminal plasma. This assay has a minimum analytical sensitivity limit of 25.0 U/ml.

Mixture of spermatozoa proteins that are recognised by anti-spermatozoa antibodies are coated on the ELISA-plate.


The minimum detection sensitivity level of human anti-spermatozoa antibodies (anti-sperm) using this human anti-sperm antibody ELISA kit was 25.0 U/ml. The dynamic assay range for this kit is 31.0 – 250.0 U/ml.


  1. A solid-phase assay for the detection of anti-sperm antibodies. Am J Reprod Immunol. (1993) 29 (4): 241-6. Okada H., et al.
  2. Naturally-occurring antisperm antibodies in men: interference with fertility and clinical implications. An update. Front Biosci. (2007) 12: 2890-911. Review. Francavilla F., et al.
  3. [A quantitative ELISA for the measurement of class-specific anti-spermatozoa antibodies]. Zentralbl Gynakol. (1990) 112 (23): 1455-61. German. Meinecke B., et al.
  4. Anti-sperm antibodies in the male: detection and clinical impact. Am J Reprod Immunol. (1992) 28 (2): 110-6. Review. Meinertz H.


  • Full Name: Anti-Spermatozoa Antibody (ASA) Seminal ELISA Kit
  • Reactivity: Human
  • Sample Type: Seminal Plasma
  • Sensitivity: 25.0 U/ml



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