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Anti-dsDNA Screen ELISA Kit

  • Created on the 31 May, 2017.

BACKGROUND

Anti-double stranded DNA (anti-dsDNA) have been vital in the diagnosis of systemic lupus erythematosus (SLE) and they are produced by the immune system when it fails to differentiate self from non-self. Majority of patient with SLE are found to display antibodies against dsDNA for IgG, IgA and IgM class within their sera, these autoantibodies are usually associated with lupus nephritis. Also, many studies have demonstrated the link of these subclass with certain parameters of the disease activity for example consumption of complement component C3, elevated erythrocyte sedimentation rate, acral necrosis, erythema, cutaneous vasculitis.

INTENDED USE

Human ds-DNA screen ELISA kit can be used for analysing in vitro quantitative levels of IgA, IgG and IgM autoantibodies to double stranded DNA (dsDNA) in human serum or plasma samples. This assay has a minimum analytical sensitivity limit of 1.0 U/ml.

SENSITIVITY

The minimum detection sensitivity level of human double stranded DNA antibodies (dsDNA screen) using this human ds-DNA screen ELISA kit was 1.0 U/ml. The dynamic assay range for this kit is 12.5 – 200.0 U/ml.

REFERENCES

  1. A recombinant topoisomerase I ELISA: screening for IgG, IgM and IgA anti-topo I autoantibodies in human sera. Clin Exp Immunol. (1992) 89 (3): 456-60. Verheijen R., et al.
  2. Significance of levels of specific immunoglobulins to DNA in SLE patients’ sera detected by solid phase radioimmunoassay. J Invest Dermatol. (1979) 72 (2): 55-8. Heinzerling R.H., et al.
  3. Analyzing pathogenic (double-stranded (ds) DNA-specific) plasma cells via immunofluorescence microscopy. Arthritis Res Ther. (2015) 17: 293. Winter O., et al.
  4. Vomitoxin-induced dysregulation of serum IgA, IgM and IgG reactive with gut bacterial and self antigens. Food Chem Toxicol. (1992) 30 (6): 499-504. Rasooly L. and Pestka J.J.

ADDITIONAL INFORMATION

  • Full Name: Anti-dsDNA Screen ELISA Kit
  • Reactivity: Human
  • Sample Type: Serum, Plasma
  • Sensitivity: 1.0 U/ml

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