Anti-dsDNA IgG ELISA Kit

  • Created on the 31 May, 2017.


Anti-dsDNA antibodies are members of the anti-nuclear antibodies (ANA) and their main target antigen is double stranded DNA. They are predominately linked to systemic lupus erythematosus (SLE) and if the disease is active then this can lead to high levels of anti-DNA antibodies being found. In patients that have lupus nephritis, a high amount of anti-dsDNA is associated with ongoing damage and inflammation to the kidneys. Patients with rheumatoid arthritis have also been found to develop double stranded DNA antibodies but this is usually tends to be related to the treatment undertaken. Many assay formats have been used to detect and quantify anti-dsDNA antibodies (such as polyethylene glycol assay, microarrays, flow cytometry, Farr assay and EIA/ELISA).


Human ds-DNA IgG ELISA kit is a procedure for measuring in vitro quantitative levels of IgG autoantibodies to double stranded DNA (dsDNA) using human plasma or serum. This assay has a minimum analytical sensitivity limit of 1.0 U/ml.


The minimum detection sensitivity level of human double stranded DNA antibodies (dsDNA IgG) using this human ds-DNA IgG ELISA kit was 1.0 U/ml. The dynamic assay range for this kit is 12.5 – 200.0 U/ml.


  1. Evaluation of a high avidity anti-dsDNA IgG enzyme-linked immunosorbent assay for the diagnosis of systemic lupus erythematosus. Int J Clin Exp Pathol. (2011) 4 (8): 748-54. Suh-Lailam B.B., et al.
  2. Violation of an evolutionarily conserved immunoglobulin diversity gene sequence preference promotes production of dsDNA-specific IgG antibodies. PLoS One. (2015) 10 (2): e0118171. Silva-Sanchez A., et al.
  3. Light chain determines the binding property of human anti dsDNA IgG autoantibodies. Biochem Biophys Res Commun. (2000) 271 (1): 240-3. Suzuki M., et al.
  4. The antigen-binding fragment of anti-double-stranded DNA IgG enhances F-actin formation in mesangial cells by binding to alpha-actinin-4. Exp Biol Med (Maywood). (2012) 237 (9): 1023-31. Zou X., et al.
  5. Significant differences in the analytic concordance between anti-dsDNA IgG antibody assays for the diagnosis of systemic lupus erythematosus–implications for inter-laboratory testing. Clin Chim Acta. (2011) 412 (11-12): 1076-80. Chiaro T.R., et al.


  • Full Name: Anti-dsDNA IgG ELISA Kit
  • Reactivity: Human
  • Sample Type: Plasma, Serum
  • Sensitivity: 1.0 U/ml



Your secretory IgA ELISA kit gave good results and I was also really impressed with how quickly we received it.

L. Johnston
PhD Student / University of Glasgow

It is refreshing to know that you have a technical team that is very knowledgeable. I have already recommended your company to other researchers in our department.

Dr. P. Anderson
Lecturer / University College London (UCL)

I am a first time user and found that your instruction manual was very easy to follow. The insulin ELISA kit performed well and I was happy with the results that were generated.

J. Thomas
Senior Technician / Addenbrooke’s Hospital

I carried out a pilot study comparing the performance of many ELISA kits from different suppliers and found your kits to be one of the better performers. We observed good linearity and tight replicates.

Dr. C. Davies
Lead Scientists / AstraZeneca

You are my first point of contact when I am looking to purchase ELISA kits. You have such an easy and simple system, yet it is very effective.

A. Shaw
Purchasing / University of Oxford