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Anti-DGP IgG ELISA Kit

  • Created on the 31 May, 2017.

BACKGROUND

Anti-gliadin antibodies are mainly produced in response to gliadin and the use of antibodies against gliadin have been vital in the diagnosis of celiac disease. There are essentially three different alleles AA, BB and DD and these can lead to a slightly different gliadins being produced in the body. The major disadvantage of using gliadin antibodies is that they are also present in individuals without celiac disease, meaning that they lack specificity even though they are quite sensitivity in comparison to measuring endomysium and transglutaminase concentrations. Recent findings have identified that gliadin-reactive antibodies from celiac patients are able to bind a limited number of specific epitopes which are locate that on the gliadin molecule. This means that the use of deamidated gliadin peptides in assay will allow a greater specificity and accuracy for determining celiac disease in comparison to the standard gliadin antibody assays. In serum samples from celiac patients both IgA and IgG antibodies against deamidated gliadin peptide epitopes have been identified. The IgG antibody is quite similar to AGA IgA, however, it can be found at higher amounts in patients with the IgA-less phenotype. There is also evidence to indicate that it is linked with non-celiac gluten sensitivity and coeliac disease, they are also found frequently with anti-transglutaminase antibodies.

INTENDED USE

Human anti-DGP IgG ELISA kit is a procedure for detecting in vitro quantitative amounts of IgG-antibodies directed against deamidated gliadin protein epitopes (DGP) in human serum and plasma samples. This assay has a minimum analytical sensitivity limit of 1.0 U/ml.

SENSITIVITY

The minimum detection sensitivity level of human deamidated gliadin protein epitopes (DGP IgG) using this human anti-DGP IgG ELISA kit was 1.0 U/ml. The dynamic assay range for this kit is 6.3 – 100.0 U/ml.

REFERENCES

  1. Diagnostic value of anti-deamidated gliadin peptide IgG antibodies for celiac disease in children and IgA-deficient patients. J Pediatr Gastroenterol Nutr. (2012) 55 (1): 50-5. Mozo L., et al.
  2. The clinical response to gluten challenge: a review of the literature. Nutrients. (2013) 5 (11): 4614-41. Review. Bruins M.J.
  3. Serological testing for celiac disease in adults. United European Gastroenterol J. (2013) 1 (5): 319-25. Schyum A.C. and Rumessen J.J.
  4. Diagnostic performance of IgG anti-deamidated gliadin peptide antibody assays is comparable to IgA anti-tTG in celiac disease. Clin Chim Acta. (2010) 411 (13-14): 931-5. Vermeersch P., et al.

ADDITIONAL INFORMATION

  • Full Name: Anti-DGP IgG ELISA Kit
  • Reactivity: Human
  • Sample Type: Serum, Plasma
  • Sensitivity: 1.0 U/ml

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