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Anti-DGP IgA ELISA Kit

  • Created on the 31 May, 2017.

BACKGROUND

Anti-gliadin antibodies can be used for diagnosing celiac disease and many other related conditions that are sensitive to gluten for example dermatitis herpetiformis. Celiac disease is an example of an autoimmune disorder where the body’s immune system mistakenly thinks that gluten (a protein in wheat, oats, rye and barley) is a foreign invader. Gliadin is a portion of the protein that is present in gluten and the immune system produces anti-gliadin antibodies (AGA) in order to attack the protein. These antibodies can be either immunoglobulin A (IgA) or immunoglobulin G (IgG), IgA is more useful in helping to detect celiac disease since it is produced in the small intestine, a region where gluten causes irritation and inflammation in sensitive people.

One of the main drawbacks for using gliadin antibodies is that they can also be found in people without celiac disease, therefore this test usually lacks specificity when compared to measuring transglutaminase and endomysium levels. However, recent research has discovered that gliadin-reactive antibodies from celiac patients have the ability to bind a limited number of specific epitopes that are present on the gliadin molecule. The use of deamidated gliadin peptides provides a means of measuring specific and accurate levels. Deamidated gliadin peptides have the ability bind to the HLA molecules of antigen-presenting cells. Once they are placed in context with specific HLA molecules, these fragments are recognised by corresponding T-helper cells. This process leads to an extensive immune response resulting in the formation of antibodies against DGP and tTG.

INTENDED USE

Human anti-DGP IgA ELISA kit can be used for measuring in vitro quantitative amounts of IgA-antibodies directed against deamidated gliadin protein epitopes (DGP) in human plasma and serum samples. This assay has a minimum analytical sensitivity limit of 1.0 U/ml.

SENSITIVITY

The minimum detection sensitivity level of human deamidated gliadin protein epitopes (DGP IgA) using this human anti-DGP IgA ELISA kit was 1.0 U/ml. The dynamic assay range for this kit is 6.3 – 100.0 U/ml.

REFERENCES

  1. Deamidated gliadin peptides as targets for celiac disease-specific antibodies. Adv Clin Chem. (2007) 44: 35-63. Review. Mothes T.
  2. Digesting all the options: laboratory testing for celiac disease. Crit Rev Clin Lab Sci. (2014) 51 (6): 358-78. Review. Barakauskas V.E., et al.
  3. Overview of biomarkers for diagnosis and monitoring of celiac disease. Adv Clin Chem. (2015) 68: 1-55. Brusca I.
  4. Deamidated gliadin peptides form epitopes that transglutaminase antibodies recognize. J Pediatr Gastroenterol Nutr. (2008) 46 (3): 253-61. Korponay-Szabó I.R., et al.
  5. [Treating coeliac disease. How do we measure adherence to the gluten-free diet?] Rev Chil Pediatr. (2016) 87 (6): 442-448. Review. Aranda E.A. and Araya M.
  6. Current trends and investigative developments in celiac disease. Immunol Invest. (2013) 42 (4): 273-84. Review. Samaşca G., et al.

ADDITIONAL INFORMATION

  • Full Name: Anti-DGP IgA ELISA Kit
  • Reactivity: Human
  • Sample Type: Plasma, Serum
  • Sensitivity: 1.0 U/ml

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