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Anti-Centromere B ELISA Kit

INTENDED USE

Human anti-centromere B ELISA kit is designed for determining in vitro quantitative concentrations of anti IgG autoantibodies against anti centromere B antibody in human serum and plasma. This assay has a minimum analytical sensitivity limit of 1.0 U/ml.

BACKGROUND

Anti-centromere antibodies (which can be also called ACAs or anti-centromere) are essential autoantibodies which are highly specific to kinetochore and centromere functions. They are usually found in limited systemic scleroderma (previously known as CREST syndrome) and sometimes in the diffuse form of scleroderma. However, these autoantibodies are quite rarely found in other rheumatic diseases or even in healthy individuals.

The detection of autoantibodies which are directed against centromere protein B has become very important for the diagnostic and prognostic of progressive systemic scleroderma (PSS). Centromere protein B is a DNA-associated protein and together with other proteins, is vital in the formation of kinetochores. It is an essential target antigen for over 95% of patients that have Raynaud’s phenomenon and scleroderma. Since majority of autoantibodies against centromere proteins are present many year before the disease appears, this makes the detection of anti-centromere B a significant indicator for the onset of scleroderma.

ANTI-CENTROMERE B ELISA KIT CONTENT

All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.

  • Divisible Microplate: Consisting of 12 modules of 8 wells each, recombinant anti centromere B is bound to microwells.
  • Calibrator A-E: Concentration 0, 10, 30, 100, 300 U/ml, made up from centromere B antibodies in a serum/buffer matrix.
  • Control Positive And Control Negative: Containing human anti-centromere B.
  • Sample Buffer (5x).
  • Enzyme Conjugate: Contains anti-human IgG antibodies, HRP labelled.
  • Substrate: Tetramethylbenzidine (TMB).
  • Stop Solution: Contains acid.
  • Wash Buffer (50x).
  • Instruction for Use.
  • Certificate of Analysis.

SENSITIVITY

The minimum detection sensitivity level of human centromere B antibody (anti centromere B) using current anti-centromere B ELISA kit was 1.0 U/ml. The dynamic range for this ELISA assay is 10.0 – 300.0 U/ml.

ASSAY CHARACTERISTICS

– Anti Centromere B: ELISA
– Sensitivity: 1.0 U/ml.
– Intra Assay: 4.4 – 5.4%
– Inter Assay: 4.2 – 5.4%

REFERENCES

  1. Sensing centromere tension: Aurora B and the regulation of kinetochore function. Trends Cell Biol. (2011) 21 (3): 133-40. Review. Lampson M.A. and Cheeseman I.M.
  2. DNA Sequence-Specific Binding of CENP-B Enhances the Fidelity of Human Centromere Function. Dev Cell. (2015) 33 (3): 314-27. Fachinetti D., et al.
  3. Discovering centromere proteins: from cold white hands to the A, B, C of CENPs. Nat Rev Mol Cell Biol. (2015) 16 (7): 443-9. Review. Earnshaw W.C.
  4. The role of CENP-B and alpha-satellite DNA: de novo assembly and epigenetic maintenance of human centromeres. Chromosome Res. (2004) 12 (6): 543-56. Review. Masumoto H., et al.
  5. Building the centromere: from foundation proteins to 3D organization. Trends Cell Biol. (2004) 14 (7): 359-68. Review. Amor D.J., et al.

ADDITIONAL INFORMATION

  • Full Name: Anti-Centromere ELISA Kit
  • Reactivity: Human
  • Sample Type: Serum, Plasma
  • Sensitivity: 1.0 U/ml

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