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Angiotensinogen Total ELISA Kit (Mouse)

  • Created on the 31 October, 2017.

BACKGROUND

Angiotensinogen (AGT) is a protein that is constitutively released into the blood where it can be cleaved in order to generate angiotensin. It belongs to the serpin family of proteins and is an a-2-globulin that is produced by the liver. It has an important role in the regulation of blood pressure and fluid balance (known as the renin-angiotensin system). Researcher have found angiotensinogen to be associated with predisposition to hypertension, heterogeneous disorders, pre-eclampsia, neonatal or foetal mortality and morbidity. The concentration of angiotensinogen in the plasma can be increased by elevating the levels of corticosteroid, angiontensin II, thyroid hormone and estrogen levels that are present in plasma.

INTENDED USE

Mouse total angiotensinogen ELISA kit is designed for detecting quantitative amounts of angiotensinogen (total) present in mouse cell culture supernatant, serum, plasma and urine samples. This assay has a minimum sensitivity detection limit of 0.03 ng/ml.

SENSITIVITY

The minimum sensitivity detection limit of angiotensinogen (total) using this mouse total angiotensinogen ELISA kit was approximately 0.03 ng/ml. The dynamic assay range for this kit is 0.16 – 10.0 ng/ml.

REFERENCES

  1. Increased cardiac angiotensin II receptors in angiotensinogen-deficient mice. Hypertension. (1998) 31 (1): 45-9. Sumida Y., et al.
  2. The adipose-tissue renin-angiotensin-aldosterone system: role in the metabolic syndrome? Int J Biochem Cell Biol. (2003) 35 (6): 807-25. Review. Engeli S., et al.
  3. Identification of renin and angiotensinogen messenger RNA sequences in mouse and rat brains. Hypertension. (1986) 8 (6): 544-8. Dzau V.J., et al.
  4. Quantification of intact plasma AGT consisting of oxidized and reduced conformations using a modified ELISA. Am J Physiol Renal Physiol. (2016) 311 (6): F1211-F1216. Satou R., et al.

ADDITIONAL INFORMATION

  • Full Name: Angiotensinogen Total ELISA Kit (Mouse)
  • Reactivity: Mouse
  • Sample Type: Serum, Urine, Plasma, Cell Culture Supernatant
  • Sensitivity: 0.03 ng/ml

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