Mouse Angiotensinogen ELISA Kit (AGT)

Full Name: Mouse Angiotensinogen ELISA Kit (AGT)
Reactivity: Mouse
Sample Type: Serum, Urine, Plasma, Cell Culture Supernatant
Sensitivity: 0.03 ng/ml

BACKGROUND

Angiotensinogen (AGT) belongs to the serpin family of proteins and is an a-2-globulin that is produced by the liver. It regulates blood pressure and fluid balance (renin-angiotensin system). By controlling these functions, it helps maintain normal homeostasis within the body. Since it controls an important function in the body, there are many supplements that have been manufactured for this protein. These supplements include natural and artificial variants of AGT. The following are some advantages of using these supplements: these protein derivatives can aid in controlling fluid balance and blood pressure. Angiotensinogen may help enhance body composition or lessen inflammation, among other health advantages. These synthetic supplements are additionally devoid of pollutants, affordable, and have no known adverse effects because they are made synthetically. A synthetic supplement is also excellent in that it can be created with precise ingredients. This is because the compound can be synthesized to have a specific target or molecule that the supplement aims to change or inhibit.

Angiotensinogen is associated with predisposition to hypertension, heterogeneous disorders, pre-eclampsia, neonatal or foetal mortality and morbidity. It is produced in the liver and adrenal glands, with a high production in pregnant women. It is triggered by the renin-angiotensin-aldosterone system (RAAS) and this causes a rise in blood pressure levels. Renin is a protease produced in the kidneys and it is mainly active when angiotensinogen binds to ACE. ACE breaks down angiotensinogen by converting it into angostatin, which inhibits the production of rennin. Angostatin also prevents the degradation of rennin. When rennin levels are blocked, there is an increased level of blood pressure due to aldosterone activity and release from the adrenal glands.

INTENDED USE

Mouse angiotensinogen ELISA kit is designed for detecting quantitative amounts of total AGT (angiotensinogen) present in mouse cell culture supernatant, serum, plasma and urine samples. This assay has a minimum sensitivity detection limit of 0.03 ng/ml.

CONTENT

All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.

Pre-Coated Plate: Anti-Mouse AGT (135).
Labeled Antibody (30x Conc.).
Standard: Recombinant.
EIA Buffer.
Solution For Labeled Antibody.
TMB Chromogen.
Stop Solution.
Wash Buffer (40x Conc.).

SENSITIVITY

The minimum sensitivity detection limit of angiotensinogen (AGT total) using this mouse angiotensinogen ELISA kit was approximately 0.03 ng/ml. The dynamic range for this assay is 0.16 – 10.0 ng/ml.

ASSAY CHARACTERISTICS

– Sensitivity: 0.03 ng/mL
– Measurement Range: 0.16 – 10 ng/mL
– Added Recovery Assay: 80.4 – 108.3%
– Intra – Assay: 3.8 – 7.0%
– Inter – Assay: 6.3 – 7.7%
– Specificity: 100%

REFERENCES

Increased cardiac angiotensin II receptors in angiotensinogen-deficient mice. Hypertension. (1998) 31 (1): 45-9. Sumida Y., et al.
The adipose-tissue renin-angiotensin-aldosterone system: role in the metabolic syndrome? Int J Biochem Cell Biol. (2003) 35 (6): 807-25. Review. Engeli S., et al.
Identification of renin and angiotensinogen messenger RNA sequences in mouse and rat brains. Hypertension. (1986) 8 (6): 544-8. Dzau V.J., et al.
Quantification of intact plasma AGT consisting of oxidized and reduced conformations using a modified ELISA. Am J Physiol Renal Physiol. (2016) 311 (6): F1211-F1216. Satou R., et al.

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