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Angiotensinogen Intact ELISA Kit (Rat)

INTENDED USE

Rat angiotensinogen intact ELISA kit is a method for quantitative detection of intact angiotensinogen (AGT) in rat serum, cell culture supernatant, EDTA-plasma and urine samples. This assay has a minimum sensitivity detection limit of 0.02 ng/ml.

BACKGROUND

Angiotensinogen (AGT) is a vital component of the renin-angiotensin system, it is constitutively produced and then released from the liver into the plasma where it can be cleaved to give angiotensin. AGT belongs to a group of protein known as serpin, it is an alpha-2-globulin protein. Angiotensinogen has been linked to pre-eclampsia, neonatal/foetal mortality, morbidity, heterogeneous disorders and predisposition to hypertension. In the blood elevating the concentration of corticosteroid, angiontensin II, estrogen and thyroid hormone can result in increasing the levels of plasma AGT.

RAT ANGIOTENSINOGEN INTACT ELISA KIT CONTENT

All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.

  • Pre-Coated Plate: Anti-Rat AGT (AI).
  • Labeled Antibody (30x Conc.).
  • Standard: Recombinant Rat Angiotensinogen.
  • EIA Buffer.
  • Solution For Labeled Antibody.
  • TMB Chromogen.
  • Stop Solution.
  • Wash Buffer (40x Conc.).

SENSITIVITY

The minimum sensitivity detection limit of angiotensinogen (AGT intact) using current rat intact angiotensinogen ELISA kit was approximately 0.02 ng/ml. The dynamic range for this assay is 0.08 – 5.0 ng/ml.

ASSAY CHARACTERISTICS

– Rat Intact Angiotensinogen: ELISA
– Sensitivity: 0.02 ng/mL
– Measurement Range: 0.08 – 5 ng/mL
– Added Recovery Assay: 86.2 – 94.2%
– Intra – Assay: 4.1 – 5.0%
– Inter – Assay: 4.3 – 5.2%
– Specificity: 100%

REFERENCES

  1. Distribution of immunoreactive angiotensin II, angiotensin I, angiotensinogen and renin in the central nervous system of intact and nephrectomized rats. Hypertension. (1984) 6 (2 Pt 2): I130-6. Healy D.P. and Printz M.P.
  2. Reduced hypertension-induced end-organ damage in mice lacking cardiac and renal angiotensinogen synthesis. J Mol Med (Berl). (2002) 80 (6): 359-66. Kang N., et al.
  3. An intact renin-angiotensin system is a prerequisite for normal renal development. J Hypertens. (2000) 18 (2): 123-37. Review. Guron G. and Friberg P.
  4. Effect of angiotensin II on angiotensinogen production in adrenalectomized rats. Life Sci. (1990) 46 (23): 1657-60. Nakamura A., et al.
  5. Hormonal control of angiotensinogen production. Life Sci. (1982) 30 (7-8): 577-84. Dzau V.J. and Herrmann H.C.
  6. Sexual dimorphism of urine angiotensinogen excretion in the rat. Jpn J Pharmacol. (1994) 64 (4): 243-50. Wang E., et al.

ADDITIONAL INFORMATION

  • Full Name: Angiotensinogen Intact ELISA Kit (Rat)
  • Reactivity: Rat
  • Sample Type: Cell Culture Supernatant, Urine, Serum, Plasma,
  • Sensitivity: 0.02 ng/ml

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