Androstenedione ELISA Kit

  • Created on the 15 February, 2017.


Human androstenedione ELISA kit is a reliable quantitative procedure for measuring androstenedione (17-Dione, 4-Androsten-3) in human serum and plasma (EDTA). This assay has a minimum sensitivity detection limit of 0.021 ng/ml.


Androstenedione (also called 4-Androsten-3, 17-Dione) is a steroid hormone which is produced by either the adrenals or the gonads, it is a very important precursor for estrone and testosterone. This gives it an important role in evaluating the functional state of these glands by simply measuring the levels of androstenedione present in serum. Androstenedione and testosterone have been known to show a high diurnal variability, where the morning sample displays the highest levels that are usually measured during the day. There are reports that indicate day by day fluctuation for androstenedione levels during the ovulatory cycle. Also, during pregnancy and at the age of puberty the serum levels of androstenedione are found to increase whilst it decreases again following menopause.

There is high circulating androstenedione levels are identified in women with 21-hydroxylase effect and polycystic ovaries. In general the overproduction is usually as a result of ovarian dysfunction or could be of adrenal origin. Whereas, a significantly reduced level of androstenedione is found in postmenopausal osteoporosis patients.


Reagents which are supplied with the androstenedione ELISA kit, please following instructions for storage conditions.
1. Microtiter Plate: polyclonal anti-androstenedione antibody coated.
2. Standard (Standard 0-5): 0 – 0.1 – 0.3 – 1.0 – 3.0 – 10 ng/mL.
3. Control Low & High.
4. Enzyme Conjugate.
5. Substrate Solution.
6. Stop Solution.
7. Wash Solution.

Note: Additional Standard 0 for sample dilution is available upon request.


The minimum sensitivity detection limit of androstenedione (17-Dione and 4-Androsten-3) using current human androstenedione ELISA kit was approximately 0.021 ng/ml. The dynamic range for this assay is 0.1 – 10.0 ng/ml.


  1. Immunoanalytical profile of androstenedione. Ann Biol Clin (Paris). (2016) 74 (4): 495-502. Doggui R.
  2. 17α-Hydroxylase (CYP17) expression and subsequent androstenedione production in the human ovary. Reprod Sci. (2010) 17 (11): 978-86. Review. Patel S.S., et al.
  3. Screening indicators of dehydroepiandosterone, androstenedione, and dihydrotestosterone use: a literature review. J Anal Toxicol. (2011) 35 (9): 638-55. Review. Shelby M.K., et al.
  4. Androgen production in women. Fertil Steril. (2002) 77 Suppl 4: S3-5. Review. Burger HG.


  • Full Name: Androstenedione ELISA Kit
  • Reactivity: Human
  • Sample Type: Serum, Plasma (EDTA)
  • Sensitivity: 0.021 ng/ml



Your secretory IgA ELISA kit gave good results and I was also really impressed with how quickly we received it.

L. Johnston
PhD Student / University of Glasgow

It is refreshing to know that you have a technical team that is very knowledgeable. I have already recommended your company to other researchers in our department.

Dr. P. Anderson
Lecturer / University College London (UCL)

I am a first time user and found that your instruction manual was very easy to follow. The insulin ELISA kit performed well and I was happy with the results that were generated.

J. Thomas
Senior Technician / Addenbrooke’s Hospital

I carried out a pilot study comparing the performance of many ELISA kits from different suppliers and found your kits to be one of the better performers. We observed good linearity and tight replicates.

Dr. C. Davies
Lead Scientists / AstraZeneca

You are my first point of contact when I am looking to purchase ELISA kits. You have such an easy and simple system, yet it is very effective.

A. Shaw
Purchasing / University of Oxford