- Created on the 15 February, 2017.
Human androstenedione ELISA kit is a reliable quantitative procedure for measuring androstenedione (17-Dione, 4-Androsten-3) in human serum and plasma (EDTA). This assay has a minimum sensitivity detection limit of 0.021 ng/ml.
Androstenedione (also called 4-Androsten-3, 17-Dione) is a steroid hormone which is produced by either the adrenals or the gonads, it is a very important precursor for estrone and testosterone. This gives it an important role in evaluating the functional state of these glands by simply measuring the levels of androstenedione present in serum. Androstenedione and testosterone have been known to show a high diurnal variability, where the morning sample displays the highest levels that are usually measured during the day. There are reports that indicate day by day fluctuation for androstenedione levels during the ovulatory cycle. Also, during pregnancy and at the age of puberty the serum levels of androstenedione are found to increase whilst it decreases again following menopause.
There is high circulating androstenedione levels are identified in women with 21-hydroxylase effect and polycystic ovaries. In general the overproduction is usually as a result of ovarian dysfunction or could be of adrenal origin. Whereas, a significantly reduced level of androstenedione is found in postmenopausal osteoporosis patients.
Reagents which are supplied with the androstenedione ELISA kit, please following instructions for storage conditions.
1. Microtiter Plate: polyclonal anti-androstenedione antibody coated.
2. Standard (Standard 0-5): 0 – 0.1 – 0.3 – 1.0 – 3.0 – 10 ng/mL.
3. Control Low & High.
4. Enzyme Conjugate.
5. Substrate Solution.
6. Stop Solution.
7. Wash Solution.
Note: Additional Standard 0 for sample dilution is available upon request.
The minimum sensitivity detection limit of androstenedione (17-Dione and 4-Androsten-3) using current human androstenedione ELISA kit was approximately 0.021 ng/ml. The dynamic range for this assay is 0.1 – 10.0 ng/ml.
- Immunoanalytical profile of androstenedione. Ann Biol Clin (Paris). (2016) 74 (4): 495-502. Doggui R.
- 17α-Hydroxylase (CYP17) expression and subsequent androstenedione production in the human ovary. Reprod Sci. (2010) 17 (11): 978-86. Review. Patel S.S., et al.
- Screening indicators of dehydroepiandosterone, androstenedione, and dihydrotestosterone use: a literature review. J Anal Toxicol. (2011) 35 (9): 638-55. Review. Shelby M.K., et al.
- Androgen production in women. Fertil Steril. (2002) 77 Suppl 4: S3-5. Review. Burger HG.
- Full Name: Androstenedione ELISA Kit
- Reactivity: Human
- Sample Type: Serum, Plasma (EDTA)
- Sensitivity: 0.021 ng/ml