GET A QUOTE

ANCA Screen ELISA Kit

  • Created on the 26 May, 2017.

BACKGROUND

Anti-neutrophil cytoplasmic antibodies (ANCA) are mostly IgG type group of autoantibodies directed towards the cytoplasmic components of neutrophil granulocytes (majority are white blood cells) and monocytes. Often found in many different autoimmune diseases associated to systemic vasculitis that can be measured using blood tests. Examples of some of the common disorders include eosinophilic granulomatosis with polyangiitis, granulomatosis with polyangiitis, drug induced vasculitides and microscopic polyangiitis. Two method used frequently to measure ANCA are ELISA and indirect immunofluorescence. With the human ANCA ELISA method it is possible to analyse specific antibodies to a particular antigen, once a positive screen has been carried out. MPO and PR3 are two of the most measured antigens.

INTENDED USE

Human ANCA ELISA kit is a method intended for screening in vitro quantitative determination of IgG class autoantibodies directed against PR3 and MPO in human serum and plasma samples. This assay has a minimum analytical sensitivity limit of diagn. 95.1%.

A mixture of purified antigens PR3 and MPO is coated on to microwells.

SENSITIVITY

The minimum detection sensitivity level of human class autoantibodies directed against PR3 or MPO (ANCA screen) using this human ANCA ELISA kit was diagn. 95.1%.The dynamic assay range for this kit is cut-off index 1.0.

REFERENCES

  1. Incidence of PR3- and MPO-ANCA autoantibody specificity changes in ANCA-associated vasculitis. Ann Clin Biochem. (2015) 52 (Pt 2): 297-301. Holding S., et al.
  2. How are antineutrophil cytoplasmic autoantibodies detected? Am J Kidney Dis. (1991) 18 (2): 154-8. Wieslander J.
  3. What do antineutrophil cytoplasmic antibodies (ANCA) tell us? Best Pract Res Clin Rheumatol. (2005) 19 (2): 263-76. Review. Savige J., et al.
  4. Audit of the clinical usefulness of a rapid qualitative ELISA screen for antimyeloperoxidase and antiproteinase 3 antibodies in the assessment of patients with suspected vasculitis. J Clin Pathol. (2003) 56 (10): 775-7. Aslam A., et al.
  5. Autoantibody detection using multiplex technologies. Lupus. (2006) 15 (7): 412-21. Review. Binder S.R.

ADDITIONAL INFORMATION

  • Full Name: ANCA Screen ELISA Kit
  • Reactivity: Human
  • Sample Type: Serum, Plasma
  • Sensitivity: 95.1%

OTHER RELATED ELISA KITS

TESTIMONIALS arrow icon

Your secretory IgA ELISA kit gave good results and I was also really impressed with how quickly we received it.

L. Johnston
PhD Student / University of Glasgow

It is refreshing to know that you have a technical team that is very knowledgeable. I have already recommended your company to other researchers in our department.

Dr. P. Anderson
Lecturer / University College London (UCL)

I am a first time user and found that your instruction manual was very easy to follow. The insulin ELISA kit performed well and I was happy with the results that were generated.

J. Thomas
Senior Technician / Addenbrooke’s Hospital

I carried out a pilot study comparing the performance of many ELISA kits from different suppliers and found your kits to be one of the better performers. We observed good linearity and tight replicates.

Dr. C. Davies
Lead Scientists / AstraZeneca

You are my first point of contact when I am looking to purchase ELISA kits. You have such an easy and simple system, yet it is very effective.

A. Shaw
Purchasing / University of Oxford