GET A QUOTE

ANA Screen ELISA Kit

  • Created on the 26 May, 2017.

BACKGROUND

Antinuclear antibodies (ANA) are present in the blood of individuals that have group of autoimmune disorders usually referred to as connective tissue diseases (CTD). They are a specific class of autoantibodies which can bind and destroy certain structures within the nucleus of the cells. ANAs have a number of different subtypes which include anti-histone, anti-sp100, anti-centromere, anti-Scl-70, anti-Ro, anti-La, anti-nRNP, anti-Sm and anti-dsDNA antibodies. They can also be found in many disorders such as rheumatoid arthritis, idiopathic thrombocytopaenic purpura, multiple sclerosis, systemic lupus erythematosus, Sjögren’s syndrome, autoimmune hepatitis, thyroid disease, scleroderma, psoriatic arthritis, discoid lupus, dermatomyositis, cancer and infections.

Initially, screening is carried out using a generic ELISA which detects human ANA of a broad specificity or indirect immunofluorescence tests. If positive, then more specific tests are carried out based on these findings. These antigen specific ELISA assays have the ability to react with single autoantigens for example Sm/RNP, SS-B/La, dsDNA, Scl-70, Sm, SS-A/Ro etc.

INTENDED USE

Human ANA screen ELISA kit is designed for screening in vitro quantitative concentrations of IgG autoantibodies to nuclear antigens in human serum or plasma. This assay has a minimum analytical sensitivity limit of diagn. 96.4%.

A mixture of purified antigens SS-A 60, SS-A 52, SS-B, RNP-70, Sm, RNP/Sm, Scl-70, Centromere B and Jo-1 is coated on to microwells.

SENSITIVITY

The minimum detection sensitivity level of human IgG autoantibodies to anti-nuclear antibodies using this human ANA ELISA kit was diagn. 96.4%.The dynamic assay range for this kit is cut-off index 1.0.

REFERENCES

  1. Performance of antinuclear antibody connective tissue disease screen. Ann N Y Acad Sci. (2007) 1109: 322-9. López-Hoyos M., et al.
  2. Evaluation of the LIAISON ANA screen assay for antinuclear antibody testing in autoimmune diseases. Ann N Y Acad Sci. (2007) 1109: 407-13. Ghillani P., et al.
  3. ANA screening: an old test with new recommendations. Ann Rheum Dis. (2010) 69 (8): 1420-2. Meroni P.L. and Schur P.H.
  4. ANA testing in the presence of acute and chronic infections. J Immunoassay Immunochem. (2016) 37 (5): 439-52. Review. Litwin C.M. and Binder S.R.
  5. Antinuclear antibody screening by ELISA and IF techniques: discrepant results in juvenile idiopathic arthritis but consistency in childhood systemic lupus erythematous. Clin Rheumatol. (2014) 33 (5): 643-7. Spârchez M., et al.

ADDITIONAL INFORMATION

  • Full Name: ANA Screen ELISA Kit
  • Reactivity: Human
  • Sample Type: Serum, Plasma
  • Sensitivity: 96.4%

OTHER RELATED ELISA KITS

TESTIMONIALS arrow icon

Your secretory IgA ELISA kit gave good results and I was also really impressed with how quickly we received it.

L. Johnston
PhD Student / University of Glasgow

It is refreshing to know that you have a technical team that is very knowledgeable. I have already recommended your company to other researchers in our department.

Dr. P. Anderson
Lecturer / University College London (UCL)

I am a first time user and found that your instruction manual was very easy to follow. The insulin ELISA kit performed well and I was happy with the results that were generated.

J. Thomas
Senior Technician / Addenbrooke’s Hospital

I carried out a pilot study comparing the performance of many ELISA kits from different suppliers and found your kits to be one of the better performers. We observed good linearity and tight replicates.

Dr. C. Davies
Lead Scientists / AstraZeneca

You are my first point of contact when I am looking to purchase ELISA kits. You have such an easy and simple system, yet it is very effective.

A. Shaw
Purchasing / University of Oxford