Alpha-Fetoprotein (AFP) ELISA Kit

  • Created on the 11 May, 2017.


Human AFP ELISA kit is a method for detecting in vitro quantitative levels of alpha-fetoprotein (alpha-fetoglobulin, alpha fetal protein or alpha-1-fetoprotein, AFP) in human serum samples. This assay has a minimum sensitivity limit of 1.78 IU/ml.


Alpha-fetoprotein (AFP) can also be called alpha-fetoglobulin, alpha-1-fetoprotein or alpha fetal protein, it is glycoprotein which is 591 amino acids long, with a molecular weight of 70KD and contains a carbohydrate moiety. AFP is most abundantly produced during fetal and neonatal development by yolk sac, liver and in small amounts through the gastrointestinal tract. The serum concentration levels of AFP rapidly decrease after birth and by the second year only trace quantities are usually detectable.

Elevated levels of serum AFP have been reported in several malignant diseases (for example primary hepatocelluar carcinoma and non-seminomatous testicular cancer). In addition, elevated serum concentrations have also been identified in patients having other noncancerous diseases such as acute viral hepatitis, ataxia telangiectasia, neonatal hyperbilirubinemia and hereditary tyrosinemia. AFP is usually measured in pregnant women in majority of cases through the analysis of amniotic fluid or maternal blood, this is a helpful test for measuring a subset of developmental abnormalities. The following alpha-fetoprotein ELISA kit is suitable for many different diagnostic applications.


The minimum detection sensitivity level of human alpha-1-fetoprotein (AFP, alpha-fetoglobulin, alpha fetal protein) using current human AFP ELISA kit was 1.78 IU/ml. The dynamic range for this assay is 10.0 – 160 IU/ml.


  1. Alpha-foetoprotein (AFP): A multi-purpose marker in hepatocellular carcinoma. Clin Chim Acta. (2016) 463: 39-44. Review. Sauzay C., et al.
  2. Successful resection of a liver metastasis from AFP-producing pancreatic cancer resulting in long-term survival: a case report and review of literature. Pancreas. (2005) 31 (4): 416-9. Natsume T., et al.
  3. Variations in normal serum alpha-fetoprotein (AFP) levels in patients with testicular cancer on surveillance. Onkologie. (2012) 35 (10): 588-91. Patel P., et al.
  4. AFP (alpha fetoprotein): who are you in gastrology? Cancer Lett. (2015) 357 (1): 43-6. Review. Sun W., et al.


  • Full Name: Alpha-Fetoprotein (AFP) ELISA Kit
  • Reactivity: Human
  • Sample Type: Serum
  • Sensitivity: 1.78 IU/ml



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