GET A QUOTE

Alpha-Fetoprotein (AFP) ELISA Kit

  • Created on the 11 May, 2017.

BACKGROUND

Alpha-fetoprotein (AFP) can also be called alpha-fetoglobulin, alpha-1-fetoprotein or alpha fetal protein, it is glycoprotein which is 591 amino acids long, with a molecular weight of 70KD and contains a carbohydrate moiety. AFP is most abundantly produced during fetal and neonatal development by yolk sac, liver and in small amounts through the gastrointestinal tract. The serum concentration levels of AFP rapidly decrease after birth and by the second year only trace quantities are usually detectable.

Elevated levels of serum AFP have been reported in several malignant diseases (for example primary hepatocelluar carcinoma and non-seminomatous testicular cancer). In addition, elevated serum concentrations have also been identified in patients having other noncancerous diseases such as acute viral hepatitis, ataxia telangiectasia, neonatal hyperbilirubinemia and hereditary tyrosinemia. AFP is usually measured in pregnant women in majority of cases through the analysis of amniotic fluid or maternal blood, this is a helpful test for measuring a subset of developmental abnormalities.

INTENDED USE

Human AFP ELISA kit is a method for detecting in vitro quantitative levels of alpha-fetoprotein (alpha-fetoglobulin, alpha fetal protein or alpha-1-fetoprotein) in human serum samples. This assay has a minimum sensitivity limit of 1.78 IU/ml.

SENSITIVITY

The minimum detection sensitivity level of human AFP (alpha-1-fetoprotein, alpha-fetoglobulin, alpha fetal protein) using this human alpha-fetoprotein ELISA kit was 1.78 IU/ml. The dynamic assay range for this kit is 10.0 – 160 IU/ml.

REFERENCES

  1. Alpha-foetoprotein (AFP): A multi-purpose marker in hepatocellular carcinoma. Clin Chim Acta. (2016) 463: 39-44. Review. Sauzay C., et al.
  2. Successful resection of a liver metastasis from AFP-producing pancreatic cancer resulting in long-term survival: a case report and review of literature. Pancreas. (2005) 31 (4): 416-9. Natsume T., et al.
  3. Variations in normal serum alpha-fetoprotein (AFP) levels in patients with testicular cancer on surveillance. Onkologie. (2012) 35 (10): 588-91. Patel P., et al.
  4. AFP (alpha fetoprotein): who are you in gastrology? Cancer Lett. (2015) 357 (1): 43-6. Review. Sun W., et al.

ADDITIONAL INFORMATION

  • Full Name: Alpha-Fetoprotein (AFP) ELISA Kit
  • Reactivity: Human
  • Sample Type: Serum
  • Sensitivity: 1.78 IU/ml

OTHER RELATED ELISA KITS

TESTIMONIALS arrow icon

Your secretory IgA ELISA kit gave good results and I was also really impressed with how quickly we received it.

L. Johnston
PhD Student / University of Glasgow

It is refreshing to know that you have a technical team that is very knowledgeable. I have already recommended your company to other researchers in our department.

Dr. P. Anderson
Lecturer / University College London (UCL)

I am a first time user and found that your instruction manual was very easy to follow. The insulin ELISA kit performed well and I was happy with the results that were generated.

J. Thomas
Senior Technician / Addenbrooke’s Hospital

I carried out a pilot study comparing the performance of many ELISA kits from different suppliers and found your kits to be one of the better performers. We observed good linearity and tight replicates.

Dr. C. Davies
Lead Scientists / AstraZeneca

You are my first point of contact when I am looking to purchase ELISA kits. You have such an easy and simple system, yet it is very effective.

A. Shaw
Purchasing / University of Oxford