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Aldosterone ELISA Kit

  • Created on the 2 February, 2017.

BACKGROUND

Aldosterone is a potent steroid hormone (mineral corticoid) that is produced by the zona glomerulosa of the adrenal cortex. One of its main function is to regulate potassium and sodium balance in the blood and in humans it is a potent mineralocorticoid. Its synthesis and release is controlled primarily through the renin-angiotensin system (RAAS), it is able to bind mineralocorticoid receptors (MR) and trigger the transcription of hormone responsive genes. In principle it is able to: increase the permeability of luminal membrane to sodium and potassium and activating the basolateral Na+/K+ pumps in the process, stimulate ATP hydrolysis, help reabsorb sodium ions and water into the blood and excrete potassium ions into the urine. Other important functions of aldosterone involve regulating acid/base balance and plasma bicarbonate (HCO3-) levels.

Chronic overproduction and secretion of aldosterone can lead to hypertension, adenomas, idiopathic and glucocorticoid-responsive hyperaldosteronism. Whereas, abnormally low levels have been known to occur in conditions such as nephropathy, renal tubular acidosis and congenital adrenal hyperplasia. Aldosterone activity is increased in Conn’s syndrome and is reduced in Addison’s disease.

INTENDED USE

Aldosterone ELISA kit is a reliable quantitative procedure for measuring aldosterone present in human serum, plasma and urine samples. This assay has a sensitivity detection limit of < 5.7 pg/ml.

SENSITIVITY

The minimum sensitivity detection limit of aldosterone using this human aldosterone ELISA kit was approximately < 5.7 pg/ml. The dynamic assay range for this kit is 20.0 – 1000.0 pg/ml.

REFERENCES

  1. Renin-Angiotensin-Aldosteron-System (RAAS). Labor und Diagnose (2005) 1406-24. Thomas L.
  2. The use of aldosterone-renin ratio as a diagnostic test for primary hyperaldosteronism and its test characteristics under different conditions of blood sampling. J Clin Endocrinol Metab. (2005) 90 (1): 72-8. Tiu S.C., et al.
  3. New aspects of rapid aldosterone signaling. Mol Cell Endocrinology (2009) 308 (1-2): 53-62. Grossmann C and Gekle M.
  4. Confirmatory tests in the diagnosis of primary aldosteronism. Horm Metab Res. (2010) 42 (6): 406-10. Mulatero P., et al.

ADDITIONAL INFORMATION

  • Full Name: Aldosterone ELISA Kit
  • Reactivity: Human
  • Sample Type: Serum, Plasma, Urine
  • Sensitivity: < 5.7 pg/ml

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