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Aflatoxin M1 ELISA Kit

  • Created on the 3 October, 2017.

BACKGROUND

Aflatoxin M1 is a metabolite of aflatoxin B1 and can be found in milk and milk related products, minor mycotoxin of Aspergillus flavus are also present in the milk of sheep and cow fed toxic meal. It is a member of the difurocoumarocyclopentenone series family, polycyclic aromatic compounds consisting of a cyclopenten-2-one ring that is fused to the difurocoumarin skeleton via a coumarin moiety. Aflatoxin M1 have been widely linked to liver damage and cancer which is the result of activating P450 to the epoxide causing the DNA to alkylate. The eventual toxicity is dependent on the balance of the liver enzymes that either activate it (Cytochrome P450) or those that detoxify it (Glutathione-S-Transferase). Aflatoxins are essentially naturally occurring mycotoxins which are be produced by a number of different species for example Aspergillus (in particular Aspergillus flavus and Aspergillus parasiticus).

INTENDED USE

Aflatoxin M1 ELISA kit is a quantitative procedure for analysing aflatoxin M1 levels present in milk or milk products. This assay has a minimum analytical sensitivity limit of < 10.0 pg/ml.

SENSITIVITY

The minimum detection sensitivity level of aflatoxin M1 using this aflatoxin M1 ELISA kit was less than 10.0 pg/ml. The standard assay range for this kit is 10.0 – 1000.0 pg/ml.

REFERENCES

  1. A competitive immunoassay with a surrogate calibrator curve for aflatoxin M1 in milk. Anal Chim Acta. (2011) 703 (1): 64-9. Guan D., et al.
  2. Development of ultrasensitive direct chemiluminescent enzyme immunoassay for determination of aflatoxin M1 in milk. Food Chem. (2014) 158: 310-4. Vdovenko M.M., et al.
  3. Detection of aflatoxin M1 in milk by dynamic light scattering coupled with superparamagnetic beads and gold nanoprobes. J Agric Food Chem. (2013) 61 (19): 4520-5. Zhang Z., et al.
  4. Comparison of ELISA, HPLC-FLD and HPLC-MS/MS Methods for Determination of Aflatoxin M1 in Natural Contaminated Milk Samples. Acta Chim Slov. (2016) 63 (4): 747-756. Kos J., et al.
  5. Direct enzyme-linked immunosorbent assayfor determining aflatoxin M1 at picogram levels in dairy products. J Assoc Off Anal Chem. (1984) 67 (6): 1098-101. Fremy J.M. and Chu F.S.

ADDITIONAL INFORMATION

  • Full Name: Aflatoxin M1 ELISA Kit
  • Sample Type: Milk, Milk products
  • Sensitivity: < 10 pg/ml

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L. Johnston
PhD Student / University of Glasgow

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Dr. P. Anderson
Lecturer / University College London (UCL)

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J. Thomas
Senior Technician / Addenbrooke’s Hospital

I carried out a pilot study comparing the performance of many ELISA kits from different suppliers and found your kits to be one of the better performers. We observed good linearity and tight replicates.

Dr. C. Davies
Lead Scientists / AstraZeneca

You are my first point of contact when I am looking to purchase ELISA kits. You have such an easy and simple system, yet it is very effective.

A. Shaw
Purchasing / University of Oxford