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ACTH ELISA Kit

INTENDED USE

Human ACTH ELISA kit is a reliable quantitative procedure for measuring adrenocorticotropic hormone (ACTH) in human plasma (EDTA) samples. This assay has a minimum sensitivity detection limit of 0.22 pg/ml.

BACKGROUND

Adrenocorticotropic hormone (ACTH) which can also be called corticotropin is a 39 amino acid peptide hormone with a molecular weight of approx. 4500 Dalton. It is secreted by corticotropes in the adenohypophysis (anterior lobe) of the pituitary gland in response to corticotropin releasing hormone (CRH) that is released from the hypothalamus. The second peptide which is able to modulate ACTH secretion is vasopressin (AVP), this stimulated by stress and can act synergistically with CRH in order to increase human ACTH secretion from the pituitary portal circulation.

Due to the fact the immunoassays are determined by antigenic structure and not biological function, this means that standard used for the following assay will react with POMC, ACTH, Pro-ACTH and even some fragments of ACTH. Adrenocorticotropic hormone can increase the synthesis and release of all adrenal steroids/androgens, cortisol and aldosterone. It is also the principal modulator of cortisol, one of the most important glucocorticoid present in humans. Biologically active ACTH can form as a result of enzymatic cleavage of a large precursor molecule, known as proopiomelanocortin (POMC). This molecule also contains within its structure the amino acid sequences of ACTH, Pro-ACTH, lipotropin, endorphin, enkephalins and ß-melanocyte stimulating hormone.

ACTH ELISA KIT CONTENT

All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.

  • Reagent 1: Biotinylated affinity purified goat antibody.
  • Reagent 2: Enzyme peroxidase labelled mouse monoclonal.
  • Wash Solution: Concentrate (Saline with surfactant).
  • Substrate: Tetramethylbenzidine (TMB).
  • Stopping solution: 1 N sulfuric acid.
  • Microplate (12 x 8-well strips): One holder containing ACTH antigen coated wells.
  • Calibrators A-F: Concentration 0-500 pg/ml, made up from ACTH.
  • Zero Calibrator: Lyophilized synthetic.
  • Controls 1 And Control 2: Containing synthetic h-ACTH (1-39).

SENSITIVITY

The minimum sensitivity detection limit of adrenocorticotropic hormone (ACTH) using current human ACTH ELISA kit was approximately 0.22 pg/ml. The dynamic range for this assay is 7.2 – 564.0 pg/ml.

ASSAY CHARACTERISTICS

– Adrenocorticotropic Hormone (ACTH): ELISA
– Intra-Assay Variation: 2.27 – 6.71%
– Inter-Assay Variation: 6.9 – 7.1%
– Recovery: 87 – 103%
– Linearity: 89 – 122%

REFERENCES

  1. Expression of adrenocorticotropic hormone, prolactin and transcriptional factors in clinically nonfunctioning pituitary adenoma. Endocr J. (2007) 54 (6): 961-8. Kageyama K., et al.
  2. An analysis of circadian rhythms in human adrenocortical secretory activity. Trans Am Clin Climatol Assoc. (1966) 77: 151-60. Liddle GW.
  3. Circadian cortisol secretory rhythms in Cushing’s disease. J Clin Endocrinol Metab. (1979) 48 (5): 760-5. Boyar R.M., et al.
  4. Circadian rhythms and levels of beta-endorphin, ACTH, and cortisol during chronic methadone maintenance treatment in humans. Life Sci. (1983) 33 Suppl 1: 409-11. Kreek M.J., et al.

ADDITIONAL INFORMATION

  • Full Name: ACTH ELISA Kit
  • Reactivity: Human
  • Sample Type: Plasma (EDTA)
  • Sensitivity: 0.22 pg/ml

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