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Adrenocorticotropic Hormone (ACTH) ELISA Kit

  • Created on the 2 February, 2017.

BACKGROUND

ACTH (adrenocorticotropic hormone) which can also be called corticotropin is a 39 amino acid peptide hormone with a molecular weight of approx. 4500 Dalton. It is secreted by corticotropes in the adenohypophysis (anterior lobe) of the pituitary gland in response to corticotropin releasing hormone (CRH) that is released from the hypothalamus. The second peptide which is able to modulate ACTH secretion is vasopressin (AVP), this stimulated by stress and can act synergistically with CRH in order to increase ACTH secretion from the pituitary portal circulation.

Adrenocorticotropic hormone can increase the synthesis and release of all adrenal steroids/androgens, cortisol and aldosterone. It is also the principal modulator of cortisol, one of the most important glucocorticoid present in humans. Biologically active ACTH can form as a result of enzymatic cleavage of a large precursor molecule, known as proopiomelanocortin (POMC). This molecule also contains within its structure the amino acid sequences of ACTH, Pro-ACTH, lipotropin, endorphin, enkephalins and ß-melanocyte stimulating hormone.

Due to the fact the immunoassays are determined by antigenic structure and not biological function, this means that standard ACTH ELISA will react with POMC, ACTH, Pro-ACTH and even some fragments of ACTH.

INTENDED USE

ACTH ELISA kit is a reliable quantitative procedure for measuring adrenocorticotropic hormone (ACTH) in human EDTA-plasma samples. This assay has a minimum sensitivity detection limit of 0.22 pg/ml.

SENSITIVITY

The minimum sensitivity detection limit of adrenocorticotropic hormone (ACTH) using this human ACTH ELISA kit was approximately 0.22 pg/ml. The dynamic assay range for this kit is 7.2 – 564.0 pg/ml.

REFERENCES

  1. Expression of adrenocorticotropic hormone, prolactin and transcriptional factors in clinically nonfunctioning pituitary adenoma. Endocr J. (2007) 54 (6): 961-8. Kageyama K., et al.
  2. An analysis of circadian rhythms in human adrenocortical secretory activity. Trans Am Clin Climatol Assoc. (1966) 77: 151-60. Liddle GW.
  3. Circadian cortisol secretory rhythms in Cushing’s disease. J Clin Endocrinol Metab. (1979) 48 (5): 760-5. Boyar R.M., et al.
  4. Circadian rhythms and levels of beta-endorphin, ACTH, and cortisol during chronic methadone maintenance treatment in humans. Life Sci. (1983) 33 Suppl 1: 409-11. Kreek M.J., et al.

ADDITIONAL INFORMATION

  • Full Name: Adrenocorticotropic Hormone (ACTH) ELISA Kit
  • Reactivity: Human
  • Sample Type: EDTA-Plasma
  • Sensitivity: 0.22 pg/ml

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