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Adrenaline (Epinephrine) ELISA Kit

  • Created on the 3 March, 2017.

BACKGROUND

Epinephrine which is also referred to as adrenaline is a hormone that is primary secreted by the medulla of the adrenal glands. Strong emotions (fear or anger) are responsible for causing the adrenaline to be released into the bloodstream. This process results in increasing the heart rate, blood pressure, sugar metabolism and muscle strength and is often referred to as the “flight or fight response”. The main function of this response is to prepare the body for strenuous activity. Epinephrine (adrenaline) is derived from tyrosine amino acid and can be sometimes referred to as a catecholamine because it contains the catechol moiety.

One of the main places in the body where epinephrine has an effect is in the liver, alongside glucagon, it is responsible for breaking down glycogen which is a form of energy storage in all animals. Adrenaline can cause the smooth muscles to relax, especially those that are contained in the lungs. It is able to bind specifically to the beta-2-adrenergic receptors on bronchiole muscle cells and this causes the bronchioles to relax, which enables intensified respiration. In heart muscle cells epinephrine is able to bind beta-adrenergic receptors which results in increasing the contraction rate of the heart, this ultimately leading to elevated levels of blood supply to the tissues within the body. In medicine it has can be used for injections in order to relieve the symptoms of allergic reactions and can also be used in cardiopulmonary resuscitation (CPR). This is a competitive ELISA assay where adrenaline (epinephrine) has been extracted through a cis-diol-specific affinity gel, acylated and then derivatized enzymatically.

INTENDED USE

Human adrenaline ELISA kit is a reliable and accurate in vitro quantitative procedure for analysing epinephrine (adrenaline) in human plasma and urine samples. This assay has a minimum sensitivity detection limit of 0.01 pg/ml (plasma) and 0.8 ng/ml (urine).

SENSITIVITY

The minimum sensitivity detection limit of epinephrine (adrenaline) using this adrenaline ELISA kit was approximately 0.8 ng/ml (urine) and 0.01 pg/ml (plasma). The dynamic assay range for this kit is 1 – 200 ng/ml.

REFERENCES

  1. Epinephrine biosynthesis: hormonal and neural control during stress. Cell Mol Neurobiol. (2006) 26 (4-6): 891-900. Review. Wong DL.
  2. Epinephrine and the metabolic syndrome. Curr Hypertens Rep. (2012) 14 (1): 1-7. Review. Ziegler M.G., et al.
  3. Stress and the adrenocortical control of epinephrine synthesis. Metabolism. (2002) 51 (6 Suppl 1): 11-4. Review. Wurtman RJ.
  4. Does nebulised adrenaline (epinephrine) reduce admission rate in bronchiolitis? Arch Dis Child. (2002) 87 (6): 548-50. Review. Meates M.
  5. Adrenaline: insights into its metabolic roles in hypoglycaemia and diabetes. Br J Pharmacol. (2016) 173 (9): 1425-37. Review. Verberne A.J., et al.

ADDITIONAL INFORMATION

  • Full Name: Adrenaline (Epinephrine) ELISA Kit
  • Reactivity: Human
  • Sample Type: Plasma, Urine
  • Sensitivity:  0.01 pg/ml (Plasma) and 0.8 ng/ml (Urine)

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