Adrenaline ELISA Kit (Epinephrine)

INTENDED USE

Human adrenaline ELISA kit is a reliable and accurate in vitro quantitative procedure for analysing adrenaline (epinephrine) in human plasma and urine samples. This assay has a minimum sensitivity detection limit of 0.01 pg/ml (P) and 0.8 ng/ml (U).

BACKGROUND

Adrenaline (also called epinephrine), is secreted by the medulla of the adrenal glands. Strong emotions (especially fear or anger) are responsible for causing it to be released into the bloodstream. This process results in increasing the heart rate, blood pressure, sugar metabolism and muscle strength, it is often referred to as the “flight or fight response”. The main function of this response is to prepare the body for strenuous activity.

Adrenaline can cause the smooth muscles to relax, especially those that are contained in the lungs. It is able to bind specifically to the beta-2-adrenergic receptors on bronchiole muscle cells and this causes the bronchioles to relax, which enables intensified respiration. In heart muscle cells epinephrine is able to bind beta-adrenergic receptors which results in increasing the contraction rate of the heart, this ultimately leading to elevated levels of blood supply to the tissues within the body.

The following kit is a competitive assay where the sample has been extracted through a cis-diol-specific affinity gel, acylated and then derivatized enzymatically.

CONTENT

All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.

• Adhesive Foil.
• Wash Buffer Concentrate (50x).
• Enzyme Conjugate – Goat anti-rabbit immunoglobulins, conjugated with peroxidase.
• TMB Chromogenic Substrate.
• Stop Solution.
• Adrenaline Microtiter Strips.
• Adrenaline Antiserum
• Adjustment Buffer.
• Standards (A-F): Concentrations 0, 1, 4, 15, 50, 200 ng/mL.
• Control 1 and Control 2.
• Acylation Buffer.
• Acylation Reagent.
• Assay Buffer.
• Coenzyme: S-adenosyl-L-methionine.
• Enzyme (Lyophilized): Catechol-O-methyltransferase.
• Extraction Buffer.
• Extraction Plate.
• Hydrochloric Acid.

SENSITIVITY

The minimum sensitivity detection limit of adrenaline (epinephrine) using current epinephrine ELISA kit was approximately 0.8 ng/ml (U) and 0.01 pg/ml (P). The dynamic range for this assay is 1 – 200 ng/ml.

ASSAY CHARACTERISTICS

– Expected Reference Value: < 20 µg/day (Urine) and < 100 pg/mL (Plasma).
– Analytical Sensitivity (Limit of Detection): 0.3 ng/mL (Urine) and 10 pg/mL.
– Analytical Specificity (Cross Reactivity): Derivatized Adrenaline (100%), Derivatized Noradrenaline (0.20%), Derivatized Dopamine (< 0.0007%), Metanephrine (0.64%), Normetanephrine (0.0009%), 3-Methoxytyramine (< 0.0007%), 3-Methoxy-4-hydroxyphenylglycol (0.03%), Tyramine (< 0.0007%), Phenylalanine, Caffeinic acid, L-Dopa, Homovanillic acid, Tyrosine, 3-Methoxy-4-hydroxymandelic acid (< 0.0007%).
– Intra Assay Precision: 6.9 – 15.0%
– Inter Assay Precision: 13.2 – 15.4%
– Linearity: 86 – 124% (Urine) and 81 – 121% (Plasma).
– Recovery: 84 – 119% (Urine) and 80 – 113% (Plasma).

REFERENCES

1. Epinephrine and the metabolic syndrome. Curr Hypertens Rep. (2012) 14 (1): 1-7. Review. Ziegler M.G., et al.
2. Stress and the adrenocortical control of epinephrine synthesis. Metabolism. (2002) 51 (6 Suppl 1): 11-4. Review. Wurtman RJ.
3. Does nebulised adrenaline (epinephrine) reduce admission rate in bronchiolitis? Arch Dis Child. (2002) 87 (6): 548-50. Review. Meates M.
4. Adrenaline: insights into its metabolic roles in hypoglycaemia and diabetes. Br J Pharmacol. (2016) 173 (9): 1425-37. Review. Verberne A.J., et al.

ADDITIONAL INFORMATION

• Full Name: Adrenaline ELISA Kit (Epinephrine)
• Reactivity: Human
• Sample Type: Plasma, Urine
• Sensitivity:  0.01 pg/ml (P) and 0.8 ng/ml (U)

OTHER RELATED ELISA KITS

• Noradrenaline (Norepinerphrine) ELISA Kit
• 5-Hydroxy-3-Indole Acetic Acid (5-HIAA) ELISA Kit
• Dopamine ELISA Kit