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Adenovirus IgG ELISA Kit

INTENDED USE

Human adenovirus IgG ELISA kit is designed for measuring in-vitro quantitative amounts of human IgG antibodies to adenovirus (adenovirus-IgG) in plasma or serum samples. This assay has a minimum analytical sensitivity limit of 1.0 U/ml.

BACKGROUND

Adenovirus is a ubiquitous pathogen which is known to be present in either animals or humans.  It is responsible for causing many different types of diseases that can either occur singly or currently. Some of the most common infections include the upper respiratory tracts, the intestinal tract and the eyes. Pneumonia is one of the dominant form of adenovirus infection that is found to occur in infants who tend to be less than one year old. There are also cases where adenoviruses can lead to swimming pool related pharyngoconjunctival fever or ketrato conjunctivitis epidemics which can affect both adults and children. In general these infections can be spread either through the aerial-droplet route or the routes which tend to be characteristic of intestinal infections, the incubation period is usually between 5-7 days.

The following adenovirus-IgG ELISA kit can be used for detecting many different types of diagnostic applications. The infections of adenovirus in majority of the cases are caused by a viral disease of the respiratory tract amongst pre-school children, where the acute diseases of the upper respiratory tract seem to be predominantly occurring. These adenoviruses can be characterised by their common location within the cell nucleus, an overall marked stability to environmental effects and being a common complement-fixing antigens. In order to accurately measure the actual infection of the virus in patients, it is recommended to carry out serological testing procedures, the preferred choice of antibody class that is frequently used during serological testing is the IgG.

ADENOVIRUS IGG ELISA KIT CONTENT

All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.

  • Adenovirus antigen coated microtiter strips.
  • Calibrator A (Negative Control).
  • Calibrator B (Cut-Off Standard).
  • Calibrator C (Weak Positive Control).
  • Calibrator D (Positive Control).
  • Enzyme Conjugate.
  • TMB Substrate.
  • Stop Solution.
  • Sample Diluent.
  • Washing Buffer (10x).

SENSITIVITY

The minimum detection sensitivity level of IgG antibodies to adenovirus (adenovirus IgG) using current human adenovirus-IgG ELISA kit was 1.0 U/ml. The dynamic range for this assay is 1.0 – 150.0 U/ml.

ASSAY CHARACTERSTICS

– Adenovirus ELISA: IgG
– Intra-Assay-Precision: 7.8 %
– Inter-Assay-Precision: 12.3 %
– Inter-Lot-Precision: 10.3 – 14.9 %
– Analytical Sensitivity: 1.00 U/mL
– Recovery: 90 – 121 %
– Linearity: 81 – 117 %
– Cross-Reactivity: No cross-reactivity to Influenza A and RSV.
– Interferences: No interferences to bilirubin up to 0.3 mg/mL, hemoglobin up to 8.0 mg/mL and triglycerides up to 5.0 mg/mL
– Clinical Specificity: 100 %
– Clinical Sensitivity: 100 %

REFERENCES

  1. Specific labelling of human rotaviruses and adenoviruses with gold-IgG complexes. J Virol Methods. (1985) 12 (1-2): 47-57. Kjeldsberg E.
  2. Antibody to human adenovirus early antigens during acute adenovirus infections. Infect Immun. (1981) 32 (2): 778-87. Gerna G., et al.
  3. [Estimation of IgG Adenovirus antibody titers using a standard curve and an indirect ultramicro ELISA essay]. Rev Cubana Med Trop. (1996) 48 (2): 102-8. Spanish. Laferté J., et al.
  4. A combined enzyme immunoassay for rotavirus and adenovirus (EIARA). J Virol Methods. (1985) 10 (1): 21-8. Pereira H.G., et al.
  5. Determination of antibodies to non-structural and structural adenovirus antigens by ELISA. Acta Virol. (1992) 36 (6): 524-32. Lähdeaho M.L., et al.

ADDITIONAL INFORMATION

  • Full Name: Adenovirus IgG ELISA Kit
  • Reactivity: Human
  • Sample Type: Serum, Plasma
  • Sensitivity: 1.0 U/ml

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