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Activin A ELISA Kit

  • Created on the 6 February, 2017.

BACKGROUND

Activin A is a 14kDa beta-A homodimer, a cytokine that is member of the transforming growth factor-beta super family. It is predominately locally expressed by mesenchymal component in hemopoietic micro-environment. The expression of activin A can be regulated by a number of different cytokines at the mRNA level, in addition there are also several inhibitory molecules which are involved in tightly controlling the biological activity of this protein.

Inhibins and activins can modulate the growth and differentiation of many cell types, they function in opposing actions inhibit (inhibins) and activate (activin) the secretion of follitropin by the pituitary gland. Inhibins and activins are both important in the regulation a many diverse functions such as; erythroid differentiation, embryonic axial development, insulin secretion, bone growth, gonadal hormone secretion, germ cell development and maturation, nerve cell survival, pituitary and hypothalamic hormone secretion and embryonic axial development. The standard product which is used in the ELISA assay is recombinant activin A that is composed of two single chains made up of 116 amino acids having a molecular mass of 26KDa.

INTENDED USE

Human activin A ELISA kit is a reliable quantitative procedure for measuring bone activin-A in human serum, cell culture supernatant, tissue lysates and body fluids. This assay has a minimum sensitivity detection limit of < 12.0 pg/ml.

SENSITIVITY

The minimum sensitivity detection limit of activin-A using this human activin-A ELISA kit was approximately < 12.0 pg/ml. The dynamic assay range for this kit is 62.5 – 4000.0 pg/ml.

REFERENCES

  1. Role of activin-A and myostatin and their signaling pathway in human myometrial and leiomyoma cell function. J Clin Endocrinol Metab. (2014) 99 (5): E775-85. Islam M.S., et al.
  2. The role of activin a in regulation of hemopoiesis. Stem Cells. (2002) 20 (6): 493-500. Shav-Tal, Y. and Zipori, D.
  3. Apoptosis modulation by activin A and follistatin in human endometrial stromal cells. Gynecol Endocrinol. (2016) 32 (2): 161-5. Coutinho L.M., et al.
  4. Human mammary gland and breast carcinoma contain immunoreactive inhibin/activin subunits: evidence for a secretion into cystic fluid. Eur J Endocrinol (1999) 141 (2): 190-4. Di, Loreto, C., et al.

ADDITIONAL INFORMATION

  • Full Name: Activin A ELISA Kit
  • Reactivity: Human
  • Sample Type: Serum, Tissue Lysate, Cell Culture Supernatant, Body Fluids
  • Sensitivity: < 12.0 pg/ml

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