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ACPA ELISA Kit

  • Created on the 26 May, 2017.

BACKGROUND

Anti-citrullinated protein antibodies (ACPA) are autoantibodies that are usually present in majority of patients which have rheumatoid arthritis (RA) and are directed against proteins and peptides that are citrullinated. The first members of this autoantibody family were discovered in the 1970s, these were anti-perinuclear factor and anti-keratin antibodies. However, later studies have demonstrated that autoantibodies from RA patients have the ability to react with a number of different citrullinated antigens such as vimentin, fibrinogen and deiminated epstein-barr virus nuclear antigen 1.

The primary diagnosis of RA in serological support and clinical manifestations has so far been restricted to the measurement of autoantibodies against rheumatoid factor (RF). This only has a moderate specificity of about 70% for RA, however, several recent studies have discovered that antibodies against citrullinated arginine residues which is present in filament proteins is found to occur in RF negative patients. Human ACPA ELISA assay displays high sensitivity and specificity for auto-antibodies against citrullinated vimentin. This is a citrullinated protein which have been identified to be in rheumatoid synovial tissue of RA patients. Also, the titer of antibodies against vimentin in RA patients shows a direct correlation with the disease activity score.

INTENDED USE

Human ACPA ELISA kit is a method intended for analysing in vitro quantitative levels of IgG antibodies against citrullinated protein (anti-citrullinated protein antibodies, ACPA) in human plasma and serum. This assay has a minimum analytical sensitivity limit of 1.0 U/ml.

SENSITIVITY

The minimum detection sensitivity level of human ACPA using this human anti-citrullinated protein antibodies ELISA kit was 1.0 U/ml. The dynamic assay range for this kit is 20.0 – 1,000.0 U/ml.

REFERENCES

  1. Genetics of ACPA-positive rheumatoid arthritis: the beginning of the end? Ann Rheum Dis. (2011) 70 Suppl 1: i51-4. Review. de Vries R.R., et al.
  2. The lung in ACPA-positive rheumatoid arthritis: an initiating site of injury? Rheumatology (Oxford). (2014) 53 (11): 1940-50. Review. Perry E., et al.
  3. The role of anticitrullinated protein antibodies in the early stages of rheumatoid arthritis. Curr Opin Rheumatol. (2016) 28 (3): 275-81. Review. Dekkers J., et al.
  4. Anti-citrullinated peptide antibody: death of the rheumatoid factor? Med J Aust. (2009) 190 (12): 693-5. Review. Chatfield S.M., et al.

ADDITIONAL INFORMATION

  • Full Name: ACPA ELISA Kit
  • Reactivity: Human
  • Sample Type: Serum, Plasma
  • Sensitivity: 1.0 U/ml

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