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8-Hydroxy-2-Deoxyguanosine ELISA Kit

INTENDED USE

Human 8-hydroxy-2-deoxyguanosine ELISA kit is an accurate in vitro quantitative method for detecting human 8-OHdG (8-hydroxy-2-deoxyguanosine, 8-OHdG check) in human saliva, serum, urine and plasma samples. This assay has a minimum sensitivity detection limit of 0.5 ng/ml.

BACKGROUND

8-hydroxy-2-deoxyguanosine (8-OHdG) is a modified base which can occur in the DNA as a result of an attack by hydroxyl radicals which are formed has either intermediates or products during the process of oxidative stress or aerobic metabolism. There are many publications which support the involvement of free radical reactions in the DNA damage process of many biomolecules which can ultimately lead to many diseases in humans for example cancer, diabetes, neurodegenerative conditions (Alzheimer’s disease), rheumatoid arthritis, atherosclerosis, inflammation, cerebral and heart ischemia-reperfusion injury. This human 8-OHdG ELISA kit is suitable for use in many different types of diagnostic applications.

The measurement of 8-OHdG levels is becoming increasingly popular as a stable, sensitive and an integral marker for oxidative damage in cellular DNA. It has been demonstrated that 8-OHdG can be excreted through urine and that its levels can be elevated if exposed to ionising radiation and tobacco smoke.

It has been found that the concentration of 8-OHdG correlates well with the damage to DNA and oxidative stress that leads to the degenerative disease states. Oxidative stress is one of the major causes of endothelial dysfunction and it might play significant roles in the pathophysiologic mechanism of erectile dysfunction. The following human 8-OHdG ELISA kit is a competitive binding assay system which uses plates that are precoated with anti 8-OHdG, the monoclonal antibody provided are in a liquid form. This means that the higher concentrations of 8-OHdG will result in a lower signal at the end of the assay procedure salivary 8-OHdG has been found to be a useful marker for oxidative damage of DNA or oxidative stress in total. There are also some evidence that the measurement of salivary 8-OHdG can be a useful biomarker for predicting hypogonadism and severe erectile dysfunction.

8-HYDROXY-2-DEOXYGUANOSINE ELISA KIT CONTENT

All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.

  • Microtiter Plate: Pre-coated with anti human 8­OHdG antibody (8×12wells, split type).
  • Primary Antibody: Contains human 8-­OHdG monoclonal antibody (clone N45.1).
  • Primary Antibody Solution.
  • Secondary Antibody: HRP­ conjugated anti mouse antibody.
  • Secondary Antibody Solution.
  • TMB Chromatic Solution.
  • Diluting Solution.
  • Washing Solution (5x).
  • Reaction Terminating Solution.
  • Standards: Concentration 0 – 200 ng/mL, made from purified human 8­OHdG antibody.
  • Plate Seal.

SENSITIVITY

The minimum sensitivity detection limit of human 8-hydroxy-2-deoxyguanosine (8-OHdG) using current human 8-OHdG ELISA kit was approximately 0.5 ng/ml. The dynamic assay range for this kit is 0.5 – 200.0 ng/ml.

REFERENCES

  1. Determination of 8-hydroxydeoxyguanosine in human cells under oxygen-free conditions. Carcinogenesis. (1996) 17 (4): 787-91. Nakajima M., et al.
  2. 8-Hydroxydeoxyguanosine as a urinary biomarker of oxidative DNA damage. J Toxicol Environ Health. (1993) 40 (2-3): 391-404. Review. Loft S., et al.
  3. Oxidative damage to mitochondrial DNA and its relationship to ageing. Int J Biochem Cell Biol. (1995) 27 (7): 647-53. Review. Richter C.
  4. Why and how should we measure oxidative DNA damage in nutritional studies? How far have we come? Am J Clin Nutr. (2000) 72 (5): 1082-7. Review. Halliwell B.
  5. Can oxidative DNA damage be used as a biomarker of cancer risk in humans? Problems, resolutions and preliminary results from nutritional supplementation studies. Free Radic Res. (1998) 29 (6): 469-86. Review. Halliwell B.

ADDITIONAL INFORMATION

  • Full Name: 8-Hydroxy-2-Deoxyguanosine ELISA Kit
  • Reactivity: Human
  • Sample Type: Saliva, Plasma, Urine, Tissue, Other Biological Samples
  • Sensitivity: 0.5 ng/ml

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