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8-Hydroxy-2’-Deoxyguanosin (8OHdG) Saliva ELISA Kit

  • Created on the 23 February, 2017.

BACKGROUND

8-hydroxydeoxyguanosine (8OHdG) is a modified base which can occur in the DNA as a result of an attack by hydroxyl radicals which are formed has either intermediates or products during the process of oxidative stress or aerobic metabolism. There are many publications which support the involvement of free radical reactions in the DNA damage process of many biomolecules which can ultimately lead to many diseases in humans for example cancer, diabetes, neurodegenerative conditions (Alzheimer’s disease), rheumatoid arthritis, atherosclerosis, inflammation, cerebral and heart ischemia-reperfusion injury.

The measurement of 8OHdG levels is becoming increasingly popular as a stable, sensitive and an integral marker for oxidative damage in cellular DNA. It has been demonstrated that 8OHdG can be excreted through urine and that its levels can be elevated if exposed to ionising radiation and tobacco smoke.

It has been found that the concentration of 8OHdG correlates well with the damage to DNA and oxidative stress that leads to the degenerative disease states. Oxidative stress is one of the major causes of endothelial dysfunction and it might play significant roles in the pathophysiologic mechanism of erectile dysfunction. This kit is a competitive binding assay system which using plates that are pre-coated with 8OHdG, the monoclonal antibody provided are in a liquid form. This means that the higher concentrations of 8OHdG will result in a lower signal at the end of the assay procedure Salivary 8OHdG has been found to be a useful marker for oxidative damage of DNA or oxidative stress in total. There are also some evidence that the measurement of salivary 8OHdG can be a useful biomarker for predicting hypogonadism and severe erectile dysfunction.

INTENDED USE

8OHdG saliva ELISA kit is an accurate in vitro quantitative method for detecting 8-hydroxydeoxyguanosine (8OHdG) in human saliva, urine, plasma, tissue and many other biological samples. This assay has a minimum sensitivity detection limit of 0.5 ng/ml.

SENSITIVITY

The minimum sensitivity detection limit of 8-hydroxydeoxyguanosine (8OHdG) using this human 8OHdG saliva ELISA kit was approximately 0.5 ng/ml. The dynamic assay range for this kit is 0.5 – 20.0 ng/ml.

REFERENCES

  1. Determination of 8-hydroxydeoxyguanosine in human cells under oxygen-free conditions. Carcinogenesis. (1996) 17 (4): 787-91. Nakajima M., et al.
  2. 8-Hydroxydeoxyguanosine as a urinary biomarker of oxidative DNA damage. J Toxicol Environ Health. (1993) 40 (2-3): 391-404. Review. Loft S., et al.
  3. Oxidative damage to mitochondrial DNA and its relationship to ageing. Int J Biochem Cell Biol. (1995) 27 (7): 647-53. Review. Richter C.
  4. Why and how should we measure oxidative DNA damage in nutritional studies? How far have we come? Am J Clin Nutr. (2000) 72 (5): 1082-7. Review. Halliwell B.
  5. Can oxidative DNA damage be used as a biomarker of cancer risk in humans? Problems, resolutions and preliminary results from nutritional supplementation studies. Free Radic Res. (1998) 29 (6): 469-86. Review. Halliwell B.

ADDITIONAL INFORMATION

  • Full Name: 8-Hydroxy-2’-Deoxyguanosin (8OHdG) Saliva ELISA Kit
  • Reactivity: Human
  • Sample Type: Saliva, Plasma, Urine, Tissue, Other Biological Samples
  • Sensitivity: 0.5 ng/ml

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