- Created on the 15 February, 2017.
Human 5alpha-DHT ELISA kit is a reliable quantitative procedure for measuring 5alpha-dihydrotestosterone (5-alpha DHT or 5α-DHT) in human serum. This assay has a minimum sensitivity detection limit of 7.3 pg/ml.
5alpha-dihydrotestosterone (5α-DHT, 5-alpha DHT) is a C19 steroid which contains possesses androgenic activity and is very similar to androstenedione and testosterone, all belonging to a family of hormones which are known as androgens. The majority of androgen are produced in the testes in the Leydig cells, they circulate the blood via binding to either albumin or sex hormone binding globulin (SHBG). There is only a small fraction of the steroid which circulates in an unbound form and this is often called the free fractions.
In females, majority of DHT is obtained from androstenedione, whereas in males approx. 70% is derived from the peripheral conversion of testosterone. The liver is the major organ which has the ability to neutralise DHT, where it undergoes a structural modifications which is a prerequisites in order to cause biological inactivation. During this process a number metabolites are formed whilst others are re turned back to circulation before undergoing renal excretion. Some clinical applications where levels of DHT are detected include: female androgenism, male pattern baldness (MPB), benign prostatic hyperplasia (BPH) and 5α-reductase (5-AR) deficiency.
Reagents which are supplied with the 5alpha DHT ELISA kit, please following instructions for storage conditions.
1. Microtiter Plate: polyclonal antibody-coated.
2. DHT-HRP Conjugate.
3. Standard A (0 pg/mL).
4. Standard B-F: 25; 100; 500; 1000; 2500 pg/mL.
5. Control Low and High.
6. TMB Substrate Solution.
7. TMB Stop Solution.
8. Wash Buffer.
9. Assay Buffer.
The minimum sensitivity detection limit of 5alpha-dihydrotestosterone (5-alpha DHT, 5α-DHT) using current human 5alpha-DHT ELISA kit was approximately 7.3 pg/ml. The dynamic range for this assay is 25.0 – 2,500.0 pg/ml.
- Regulation of aromatase gene expression in Leydig cells and germ cells. J Steroid Biochem Mol Biol. (2003) 86 (3-5): 335-43. Review. Bourguiba S., et al.
- The accumulation of 5alpha-dihydrotesterone (5alpha-DHT) by human breast tissue. J Steroid Biochem. (1977) 8 (1): 105-6. Deshpander N., et al.
- Identification of the molecular switch that regulates access of 5alpha-DHT to the androgen receptor. Mol Cell Endocrinol. (2007) 265-266: 77-82. Review. Penning T.M., et al.
- Androgen receptors in hormone-dependent and castration-resistant prostate cancer. Pharmacol Ther. (2013) 140 (3): 223-38. Review. Shafi A.A., Yen A.E. and Weigel N.L.
- Full Name: 5alpha-Dihydrotestosterone (5alpha-DHT) ELISA Kit
- Reactivity: Human
- Sample Type: Serum
- Sensitivity: 7.3 pg/ml