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3-CAT (Adrenaline/Noradrenaline/Dopamine) Research ELISA Kit

  • Created on the 3 March, 2017.

BACKGROUND

Adrenalin, noradrenalin and dopamine are found to be synthesised in the adrenal medulla, the sympathetic nervous system and in the brain. They are all catecholamines that have the ability to have an effect on every tissue, also they can interact with other hormonal and neuronal systems to regulate many different physiological processes. They are becoming increasing used as vital diagnostic markers for many diseases, since greater amounts of catecholamines and their metabolites (metanephrine  and normetanephrine) are being secreted in large quantities in these diseases. There has been a particular focus in tumor diseases that are linked with the nervous system such as ganglioneuroma, pheochromocytoma and neuroblastoma.

This ELISA assay is based on a competitive procedure and the adrenaline (epinephrine), noradrenaline (norepinephrine) and dopamine have all being been extracted using a cis-diol-specific affinity gel, acylated and then enzymatically derivatized. Since the chemical structure of catecholamines is identical in all animals and the fact that an extraction step has been performed this allows the kit to be used for a wide range of animal species.

INTENDED USE

3-CAT (epinephrine/norepinephrine/dopamine) ELISA kit is a procedure which can accurately measure in vitro quantitative levels of adrenaline (epinephrine), noradrenaline (norepinephrine) and dopamine in EDTA-plasma, cell culture and many other biological sample types. This assay has a minimum sensitivity detection limit of 0.1 pg/ml for NAD and 0.25 pg/ml for AD and DOP.

SENSITIVITY

The minimum detection levels of adrenaline (epinephrine), noradrenaline (norepinephrine) and dopamine using this 3-CAT (epinephrine/norepinephrine/dopamine) ELISA kit was approximately 0.25 ng/ml for AD/DOP and 0.1 ng/ml NAD. The dynamic assay range for this kit is AD/DOP: 0.5 – 80 ng/ml and NAD: 0.2 – 32 ng/ml.

REFERENCES

  1. Recent advances in methods for the analysis of catecholamines and their metabolites. Anal Bioanal Chem. (2006) 386 (3): 506-14. Review. Tsunoda M.
  2. Identifying links in the chain: the dynamic coupling of catecholamines, peptide synthesis, and peptide release in hypothalamic neuroendocrine neurons. Adv Pharmacol. (2013) 68: 421-44. Review. Watts A.G. and Khan A.M.
  3. Catecholamines and stress. Ideggyogy Sz. (2014) 67 (3-4): 116-20. Review. Palkovits M.
  4. Recent insights on circulating catecholamines in hypertension. Curr Hypertens Rep. (2014) 16 (12): 498. Review. Ton Q.V. and Hammes S.R.
  5. Neurotransmitters as regulators of tumor angiogenesis and immunity: the role of catecholamines. J Neuroimmune Pharmacol. (2013) 8 (1): 7-14. Review. Sarkar C., et al.
  6. Identifying links in the chain: the dynamic coupling of catecholamines, peptide synthesis, and peptide release in hypothalamic neuroendocrine neurons. Adv Pharmacol. (2013) 68: 421-44. Review. Watts A.G. and Khan A.M.

ADDITIONAL INFORMATION

  • Full Name: 3-CAT (Adrenaline/Noradrenaline/Dopamine) Research ELISA Kit
  • Reactivity: Any Species
  • Sample Type: EDTA-Plasma, Cell Culture, Other Biological Samples
  • Sensitivity:  AD/DOP: 0.25 ng/ml and NAD: 0.1 ng/ml

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