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3-CAT (Adrenaline/Noradrenaline/Dopamine) ELISA Kit

  • Created on the 3 March, 2017.

BACKGROUND

Adrenalin, noradrenalin and dopamine are all catecholamines which are synthesised in the adrenal medulla, the sympathetic nervous system and in the brain. They are found to known to effect virtually every tissue in the human body and can also work together with other neuronal and hormonal systems in order to regulate a wide range of physiological processes. Catecholamines and their metabolites (normetanephrine and metanephrine) have become useful markers for diagnostic purposes since they are secreted in greater amounts in a large number of diseases. In particular tumor diseases which are associated with the nervous system are of primary importance, examples include neuroblastoma, pheochromocytoma and ganglioneuroma.

This is a competitive ELISA assay where adrenaline (epinephrine), noradrenaline (norepinephrine) and dopamine have been extracted using a cis-diol-specific affinity gel, acylated and then derivatized enzymatically. Due to the fact that an extraction step has been performed, this allows the kit to be used for a large number of animal species since the chemical structure of these catecholamines is identical in all animals.

INTENDED USE

3-CAT (epinephrine/norepinephrine/dopamine) ELISA kit is an accurate and reliable in vitro quantitative method for detecting adrenaline (epinephrine), noradrenaline (norepinephrine) and dopamine in plasma and urine. This assay has a minimum sensitivity detection limit of 0.01 pg/ml (plasma) or 0.8 ng/ml (urine) for AD, 0.04 pg/ml (plasma) or 1.7 ng/ml (urine) for NAD and 1.9 pg/ml (plasma) or 2.5 ng/ml (urine) for DOP.

SENSITIVITY

The minimum sensitivity detection limit of adrenaline (epinephrine), noradrenaline (norepinephrine) and dopamine using this 3-CAT (epinephrine/norepinephrine/dopamine) ELISA kit was approximately 0.3 ng/ml (urine) or 10 pg/ml (plasma) for AD, 1.5 ng/ml (urine) or 50 pg/ml (plasma) for NAD, and 2.5 ng/ml (urine) or 1.9 pg/ml (plasma) for DOP. The dynamic assay range for this kit is AD: 1 – 200 ng/ml, NAD: 5 – 1,000 ng/ml and DOP: 10 – 2,000 ng/ml.

REFERENCES

  1. A new approach to reducing postsurgical cancer recurrence: perioperative targeting of catecholamines and prostaglandins. Clin Cancer Res. (2012) 18 (18): 4895-902. Review. Neeman E, et al.
  2. Norepinephrine and dopamine as learning signals. Neural Plast. (2004) 11 (3-4): 191-204. Review. Harley CW.
  3. Relevance of norepinephrine-dopamine interactions in the treatment of major depressive disorder. CNS Neurosci Ther. (2010) 16 (3): e1-17. Review. El Mansari M, et al.
  4. Determination of catecholamines in plasma and urine. Best Pract Res Clin Endocrinol Metab. (2013) 27 (5): 713-23. Review. Grouzmann E. and Lamine F.

ADDITIONAL INFORMATION

  • Full Name: 3-CAT (Adrenaline/Noradrenaline/Dopamine) ELISA Kit
  • Reactivity: Any Species
  • Sample Type: Plasma, Urine
  • Sensitivity:  AD: 0.3 ng/ml (Urine); 10 pg/ml (Plasma) and NAD: 1.5 ng/ml (Urine); 50 pg/ml (Plasma) and DOP: 2.5 ng/ml (Urine); 1.9 pg/ml (Plasma)

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