3-CAT (Adrenaline/Noradrenaline/Dopamine) ELISA Kit

  • Created on the 3 March, 2017.


Adrenalin, noradrenalin and dopamine are all catecholamines which are synthesised in the adrenal medulla, the sympathetic nervous system and in the brain. They are found to known to effect virtually every tissue in the human body and can also work together with other neuronal and hormonal systems in order to regulate a wide range of physiological processes. Catecholamines and their metabolites (normetanephrine and metanephrine) have become useful markers for diagnostic purposes since they are secreted in greater amounts in a large number of diseases. In particular tumor diseases which are associated with the nervous system are of primary importance, examples include neuroblastoma, pheochromocytoma and ganglioneuroma.

This is a competitive ELISA assay where adrenaline (epinephrine), noradrenaline (norepinephrine) and dopamine have been extracted using a cis-diol-specific affinity gel, acylated and then derivatized enzymatically. Due to the fact that an extraction step has been performed, this allows the kit to be used for a large number of animal species since the chemical structure of these catecholamines is identical in all animals.


3-CAT (epinephrine/norepinephrine/dopamine) ELISA kit is an accurate and reliable in vitro quantitative method for detecting adrenaline (epinephrine), noradrenaline (norepinephrine) and dopamine in plasma and urine. This assay has a minimum sensitivity detection limit of 0.01 pg/ml (plasma) or 0.8 ng/ml (urine) for AD, 0.04 pg/ml (plasma) or 1.7 ng/ml (urine) for NAD and 1.9 pg/ml (plasma) or 2.5 ng/ml (urine) for DOP.


The minimum sensitivity detection limit of adrenaline (epinephrine), noradrenaline (norepinephrine) and dopamine using this 3-CAT (epinephrine/norepinephrine/dopamine) ELISA kit was approximately 0.3 ng/ml (urine) or 10 pg/ml (plasma) for AD, 1.5 ng/ml (urine) or 50 pg/ml (plasma) for NAD, and 2.5 ng/ml (urine) or 1.9 pg/ml (plasma) for DOP. The dynamic assay range for this kit is AD: 1 – 200 ng/ml, NAD: 5 – 1,000 ng/ml and DOP: 10 – 2,000 ng/ml.


  1. A new approach to reducing postsurgical cancer recurrence: perioperative targeting of catecholamines and prostaglandins. Clin Cancer Res. (2012) 18 (18): 4895-902. Review. Neeman E, et al.
  2. Norepinephrine and dopamine as learning signals. Neural Plast. (2004) 11 (3-4): 191-204. Review. Harley CW.
  3. Relevance of norepinephrine-dopamine interactions in the treatment of major depressive disorder. CNS Neurosci Ther. (2010) 16 (3): e1-17. Review. El Mansari M, et al.
  4. Determination of catecholamines in plasma and urine. Best Pract Res Clin Endocrinol Metab. (2013) 27 (5): 713-23. Review. Grouzmann E. and Lamine F.


  • Full Name: 3-CAT (Adrenaline/Noradrenaline/Dopamine) ELISA Kit
  • Reactivity: Any Species
  • Sample Type: Plasma, Urine
  • Sensitivity:  AD: 0.3 ng/ml (Urine); 10 pg/ml (Plasma) and NAD: 1.5 ng/ml (Urine); 50 pg/ml (Plasma) and DOP: 2.5 ng/ml (Urine); 1.9 pg/ml (Plasma)



Your secretory IgA ELISA kit gave good results and I was also really impressed with how quickly we received it.

L. Johnston
PhD Student / University of Glasgow

It is refreshing to know that you have a technical team that is very knowledgeable. I have already recommended your company to other researchers in our department.

Dr. P. Anderson
Lecturer / University College London (UCL)

I am a first time user and found that your instruction manual was very easy to follow. The insulin ELISA kit performed well and I was happy with the results that were generated.

J. Thomas
Senior Technician / Addenbrooke’s Hospital

I carried out a pilot study comparing the performance of many ELISA kits from different suppliers and found your kits to be one of the better performers. We observed good linearity and tight replicates.

Dr. C. Davies
Lead Scientists / AstraZeneca

You are my first point of contact when I am looking to purchase ELISA kits. You have such an easy and simple system, yet it is very effective.

A. Shaw
Purchasing / University of Oxford