GET A QUOTE

2-CAT (Adrenaline/Noradrenaline) Research ELISA Kit

  • Created on the 3 March, 2017.

BACKGROUND

Adrenaline (also called epinephrine) is mainly functioning as a hormone and is formed in the adrenal medulla, however, there are small amounts of it which act as neurotransmitter as they are produced in nerve fibres. Whereas, noradrenaline (also known as norepinephrine) is a most common neurotransmitter and is also a hormone. Both adrenaline and noradrenaline are catecholamines.

Epinephrine is able to act on almost every body tissue but can have varying effects depending on which tissue it is acting on. It is mostly produced from norepinephrine from the adrenal medulla. It has been well documented that the synthesis of epinephrine is elevated during times of stress (physical and emotional).

Norepinephrine is mainly produced from nerve axons (shaft of the nerve) and it is stored in small fluid-filled sacs which are called vesicles. During an action potential (usually an electrical pulse), it is released and can travel down the nerve and across the gap between two nerves where it binds to a receptor on the second nerve, this action stimulates the nerve to respond. This action is useful in causing vasoconstriction which is vital in either maintaining or increasing blood pressure.

This is a flexible competitive ELISA assay system that can be used for number of different biological sample types and volumes. The epinephrine (adrenaline) and norepinephrine (noradrenaline) are both extracted by using a cis-diol-specific affinity gel, acylated and then converted enzymatically.

INTENDED USE

2-CAT (epinephrine/norepinephrine) ELISA kit is a reliable and accurate in vitro quantitative procedure for analysing epinephrine (adrenaline) and norepinephrine (noradrenaline) in cell culture, EDTA-plasma and many other biological samples types. This assay has a minimum sensitivity detection limit of 0.25 ng/ml (AD) and 0.1 ng/ml (NAD).

SENSITIVITY

The minimum sensitivity detection limit of epinephrine (adrenaline) and norepinephrine (noradrenaline) using this 2-CAT (epinephrine/norepinephrine) ELISA kit was approximately 0.1 ng/ml (NAD) and 0.25 ng/ml (AD). The dynamic assay range for this kit is AD: 0.5 – 80 ng/ml and NAD: 0.2 – 32 ng/ml.

REFERENCES

  1. Peripheral and central effects of circulating catecholamines. Compr Physiol. (2015) 5 (1): 1-15. Review. Tank A.W. and Lee Wong D.
  2. Norepinephrine: more of a neurohormone than a vasopressor. Crit Care. (2010) 14 (5): 196. Review. Myburgh J.
  3. Ficts and facts of epinephrine and norepinephrine stability in injectable solutions. Int J Pharm. (2012) 434 (1-2): 468-80. Review. Hoellein L. and Holzgrabe U.
  4. Cardiac arrest: should we consider norepinephrine instead of epinephrine? Am J Emerg Med. (2014) 32 (12): 1560.e1-2. Mion G., et al.

ADDITIONAL INFORMATION

  • Full Name: 2-CAT (Adrenaline/Noradrenaline) Research ELISA Kit
  • Reactivity: Any Species
  • Sample Type: EDTA-Plasma, Cell Culture, Other Biological Samples
  • Sensitivity:  AD: 0.25 ng/ml and NAD: 0.1 ng/ml

OTHER RELATED ELISA KITS

TESTIMONIALS arrow icon

Your secretory IgA ELISA kit gave good results and I was also really impressed with how quickly we received it.

L. Johnston
PhD Student / University of Glasgow

It is refreshing to know that you have a technical team that is very knowledgeable. I have already recommended your company to other researchers in our department.

Dr. P. Anderson
Lecturer / University College London (UCL)

I am a first time user and found that your instruction manual was very easy to follow. The insulin ELISA kit performed well and I was happy with the results that were generated.

J. Thomas
Senior Technician / Addenbrooke’s Hospital

I carried out a pilot study comparing the performance of many ELISA kits from different suppliers and found your kits to be one of the better performers. We observed good linearity and tight replicates.

Dr. C. Davies
Lead Scientists / AstraZeneca

You are my first point of contact when I am looking to purchase ELISA kits. You have such an easy and simple system, yet it is very effective.

A. Shaw
Purchasing / University of Oxford