- Created on the 3 March, 2017.
2-CAT (adrenaline/noradrenaline) research ELISA kit is a reliable and accurate in vitro quantitative procedure for analysing epinephrine (adrenaline) and norepinephrine (noradrenaline) in cell culture, EDTA-plasma and many other biological samples types. This assay has a minimum sensitivity detection limit of 0.25 ng/ml (AD) and 0.1 ng/ml (NAD).
Adrenaline (also called epinephrine) is mainly functioning as a hormone and is formed in the adrenal medulla, however, there are small amounts of it which act as neurotransmitter as they are produced in nerve fibres. Whereas, noradrenaline (also known as norepinephrine) is a most common neurotransmitter and is also a hormone. Both adrenaline and noradrenaline are catecholamines.
Epinephrine is able to act on almost every body tissue but can have varying effects depending on which tissue it is acting on. It is mostly produced from norepinephrine from the adrenal medulla. It has been well documented that the synthesis of epinephrine is elevated during times of stress (physical and emotional).
Norepinephrine is mainly produced from nerve axons (shaft of the nerve) and it is stored in small fluid-filled sacs which are called vesicles. During an action potential (usually an electrical pulse), it is released and can travel down the nerve and across the gap between two nerves where it binds to a receptor on the second nerve, this action stimulates the nerve to respond. This action is useful in causing vasoconstriction which is vital in either maintaining or increasing blood pressure.
The following 2-CAT (adrenaline/noradrenaline) research ELISA kit is a flexible competitive assay system that can be used for number of different biological sample types and volumes. The epinephrine (adrenaline) and norepinephrine (noradrenaline) are both extracted by using a cis-diol-specific affinity gel, acylated and then converted enzymatically.
The minimum sensitivity detection limit of adrenaline (epinephrine) and noradrenaline (norepinephrine) using current 2-CAT (adrenaline/noradrenaline) research ELISA kit was approximately 0.1 ng/ml (NAD) and 0.25 ng/ml (AD). The dynamic range for this assay is AD: 0.5 – 80 ng/ml and NAD: 0.2 – 32 ng/ml.
- Peripheral and central effects of circulating catecholamines. Compr Physiol. (2015) 5 (1): 1-15. Review. Tank A.W. and Lee Wong D.
- Norepinephrine: more of a neurohormone than a vasopressor. Crit Care. (2010) 14 (5): 196. Review. Myburgh J.
- Ficts and facts of epinephrine and norepinephrine stability in injectable solutions. Int J Pharm. (2012) 434 (1-2): 468-80. Review. Hoellein L. and Holzgrabe U.
- Cardiac arrest: should we consider norepinephrine instead of epinephrine? Am J Emerg Med. (2014) 32 (12): 1560.e1-2. Mion G., et al.
- Full Name: 2-CAT (Adrenaline/Noradrenaline) Research ELISA Kit
- Reactivity: Any Species
- Sample Type: EDTA-Plasma, Cell Culture, Other Biological Samples
- Sensitivity: AD: 0.25 ng/ml and NAD: 0.1 ng/ml