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17-OH Progesterone ELISA Kit

  • Created on the 15 February, 2017.

BACKGROUND

17-hydroxyprogesterone (17-OH progesterone or 17OHP) can be derived either from progesterone via 17-hydroxylase using the enzyme P450c17 or from 17-hydroxypregnenolone using the enzymes 3β-hydroxy-steroid dehydrogenase/delta 5-4 isomerase. It is a C-21 steroid hormone that can be produced during the synthesis of sex steroids (in the gonads) and glucocorticoids (in the adrenal gland). 17-OH progesterone has been routinely used as a suitable biochemical marker for diagnosing congenital adrenal hyperplasia (CAH). Deficiencies in 21-hydroxylase are found frequently in patients that have congenital adrenal hyperplasia and this result in excessive secretion of 17-OHP causing the blood levels to be elevated. Therefore, by measuring the activity of 21-hydroxylase in the adrenal cortex can be used to monitor the levels of 17-OHP that are present in the blood and this in turn plays a significant role when diagnosing patients with congenital adrenal hyperplasia (CAH), since the typical defective enzymes (21-hydroxylase and 11b-hydroxilase) lead to the build-up of 17-hydroxyprogesterone (17OHP). CAH is one of the most frequently inborn endocrine disorders, where a prevalence of 1-20 in 10,000 children can be experienced.

17-hydroxyprogesterone is a natural progestin and is found to increase during pregnancy in the third trimester which is predominately caused by the increase in production of fetal adrenal. Generally, normal levels in children range between 3-90 ng/dl and in women between 15-70 ng/dl prior to ovulation and these increase to 35-290 ng/dl during the luteal phase of the menstrual cycle.

INTENDED USE

Human 17-OH progesterone ELISA kit is a reliable quantitative procedure for measuring 17-hydroxyprogesterone (17OHP) in human serum and plasma. This assay has a minimum sensitivity detection limit of 0.037 ng/ml.

SENSITIVITY

The minimum sensitivity detection limit of 17-hydroxyprogesterone (17OHP) using this human 17-OH progesterone ELISA kit was approximately 0.037 ng/ml. The dynamic assay range for this kit is 0.1 – 25.0 ng/ml.

REFERENCES

  1. 17-Hydroxyprogesterone in children, adolescents and adults. Ann Clin Biochem. (2014) 51 (Pt 4): 424-40. Honour J.W.
  2. Quantitation of 17-OH-progesterone (OHPG) for diagnosis of congenital adrenal hyperplasia (CAH). Methods Mol Biol. (2010) 603: 271-7. Singh R.J.
  3. Circulating progesterone and obesity in men. Horm Metab Res. (2006) 38 (5): 330-5. Blanchette S., et al.
  4. Neonatal screening for congenital adrenal hyperplasia. Nat Rev Endocrinol. (2009) 5 (9): 490-8. Review. White P.C.

ADDITIONAL INFORMATION

  • Full Name: 17-OH Progesterone ELISA Kit
  • Reactivity: Human
  • Sample Type: Serum, Plasma
  • Sensitivity: 0.037 ng/ml

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