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17 Beta Estradiol ELISA Kit

INTENDED USE

Human 17 beta estradiol ELISA kit is a reliable quantitative procedure for measuring 17-beta estradiol (17β-estradiol, 17 beta estradiol, 17b-diol) in human serum or plasma samples. This assay has a minimum sensitivity detection limit of 8.68 pg/ml.

BACKGROUND

17 beta estradiol (17β-estradiol, 17b-diol or 17-beta estradiol) is a sex hormone which represents the most important natural estrogen in humans. It has a major role in reproduction and sexual functioning, there is also evidence to indicate that it can affect many other organs which include bone structure. Most of the estradiol in women is produced by the ovaries (only a small amount by the adrenal cortex), whereas in men it is produced in the testes. In females, estradiol is known to act as a growth hormone for tissue that are involved in the reproductive organs and in males it is involved with fertility. In adult pre-menopausal women, the production of estradiol by the ovaries can be stimulated by follicle-stimulating hormone (FSH) and luteinizing hormone (LH) during the menstrual cycle. The measurement of estradiol levels can be used to evaluate fertility, monitor the ovarian follicular function during the induction phase of ovulation and detect any possible menstrual irregularities that may exist. During pregnancy the levels of estrogen which also includes estradiol are found to gradually increase towards term, the reason for this increase is due to placental production.

In plasma only a small faction is free (the biologically active form) which is approx. 1-3% whereas majority of it is found is bound to sex hormone binding globulin (SHBG) or albumin. It is only the free form that represents the active molecule during endocrine regulation. There is evidence to suggest that 17beta estradiol affects the production of many proteins which includes: binding proteins, lipoproteins, blood clotting proteins and neuroprotective proteins. It has also been shown to be involved with some types of cancers such as uterine lining, breast and many benign gynaecologic conditions (leiomyomata uteri, uterine bleeding and endometriosis).

17 BETA ESTRADIOL ELISA KIT CONTENT

All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.

  • Microtiter Plate: 12 x 8 (break apart) strips with 96 wells; Wells coated with 17β estradiol antigen.
  • Calibrator 0-5: Concentrations: 0-2000 pg/ml, made up from anti 17-beta estradiol.
  • Control 1 (Low) and Control 2 (High): Containing human 17beta estradiol.
  • Enzyme Conjugate: Consisting of  17-beta estradiol labeled horseradish peroxidase in buffered matrix.
  • ubstrate Solution: Tetramethylbenzidine (TMB).
  • Stop Solution: Contains 2 N hydrochloric acid solution.
  • Wash Solution (10x).

SENSITIVITY

The minimum sensitivity detection limit of 17 beta estradiol (17β-estradiol, 17b-diol) using this human 17-beta estradiol ELISA kit was approximately 8.68 pg/ml. The dynamic assay range for this assay is 25.0 – 2000.0 pg/ml.

REFERENCES

  1. Free estradiol and sex hormone-binding globulin. Steroids. (2015) 99 (Pt A): 113-6. Review. Rosner W.
  2. The role of estradiol in male reproductive function. Asian J Androl. (2016) 18 (3): 435-40. Review. Schulster M., et al.
  3. The effects of estradiol on mood and behavior in human female adolescents: a systematic review. Eur J Pediatr. (2015) 174 (3): 289-98. Review. Balzer B.W., et al.
  4. Estradiol assays–The path ahead. Steroids. (2015) 99 (Pt A): 39-44. Review. Ketha H., Girtman A. and Singh R.J.
  5. Correlation between oestradiol and progesterone in cycles with luteal phase deficiency, Fertil. Steril. (1982) 37: 348-54. Goldstein D., et al.

ADDITIONAL INFORMATION

  • Full Name: 17 Beta Estradiol ELISA Kit
  • Reactivity: Human
  • Sample Type: Serum, Plasma
  • Sensitivity: 8.68 pg/ml

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