17-Beta Estradiol ELISA Kit

  • Created on the 15 February, 2017.


Human 17-beta estradiol ELISA kit is a reliable quantitative procedure for measuring 17-beta estradiol (17β-estradiol or 17b-diol) in human serum or plasma samples. This assay has a minimum sensitivity detection limit of 8.68 pg/ml.


17-beta estradiol (17β-estradiol, 17b-diol or E2) is a sex hormone which represents the most important natural estrogen in humans. It has a major role in reproduction and sexual functioning, there is also evidence to indicate that it can affect many other organs which include bone structure. Most of the estradiol in women is produced by the ovaries (only a small amount by the adrenal cortex), whereas in men it is produced in the testes. In females, estradiol is known to act as a growth hormone for tissue that are involved in the reproductive organs and in males it is involved with fertility. In adult pre-menopausal women, the production of estradiol by the ovaries can be stimulated by follicle-stimulating hormone (FSH) and luteinizing hormone (LH) during the menstrual cycle. The measurement of estradiol levels can be used to evaluate fertility, monitor the ovarian follicular function during the induction phase of ovulation and detect any possible menstrual irregularities that may exist. During pregnancy the levels of estrogen which also includes estradiol are found to gradually increase towards term, the reason for the increase in estradiol is due to placental production.

There is evidence to suggest that estradiol affects the production of many proteins which includes: binding proteins, lipoproteins, blood clotting proteins and neuroprotective proteins. It has also been shown to be involved with some types of cancers such as uterine lining, breast and many benign gynaecologic conditions (leiomyomata uteri, uterine bleeding and endometriosis). Using 17-beta estradiol ELISA kit to measure 17b estradiol levels can be useful in men for differential diagnosis of gynecomastia whereas in girls it can be used to diagnosis of pubertas praecox. In plasma only a small faction is free (the biologically active form) which is approx. 1-3% whereas majority of it is found is bound to sex hormone binding globulin (SHBG) or albumin. It is only the free form that represents the active molecule during endocrine regulation.


The minimum sensitivity detection limit of 17beta estradiol (17β-estradiol) using this human 17-beta estradiol ELISA kit was approximately 8.68 pg/ml. The dynamic assay range for this assay is 25.0 – 2000.0 pg/ml.


  1. Free estradiol and sex hormone-binding globulin. Steroids. (2015) 99 (Pt A): 113-6. Review. Rosner W.
  2. The role of estradiol in male reproductive function. Asian J Androl. (2016) 18 (3): 435-40. Review. Schulster M., et al.
  3. The effects of estradiol on mood and behavior in human female adolescents: a systematic review. Eur J Pediatr. (2015) 174 (3): 289-98. Review. Balzer B.W., et al.
  4. Estradiol assays–The path ahead. Steroids. (2015) 99 (Pt A): 39-44. Review. Ketha H., Girtman A. and Singh R.J.
  5. Correlation between oestradiol and progesterone in cycles with luteal phase deficiency, Fertil. Steril. (1982) 37: 348-54. Goldstein D., et al.


  • Full Name: 17-Beta Estradiol ELISA Kit
  • Reactivity: Human
  • Sample Type: Serum, Plasma
  • Sensitivity: 8.68 pg/ml




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